Esculin, a Coumarin Glucoside Prevents Fluoride-Induced Oxidative Stress and Cardiotoxicity in Zebrafish Larvae.

IF 4.4 3区 医学 Q2 ENVIRONMENTAL SCIENCES
Srija Babu, Naveen Surya Velmani, Suryaa Manoharan, Ekambaram Perumal
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引用次数: 0

Abstract

Fluoride (F-) is a major groundwater contaminant spread across the world. In excess concentrations, F- can be detrimental to living beings. F- exposure is linked to cellular redox dyshomeostasis, leading to oxidative stress-mediated pathologies including heart dysfunction. Due to its potent antioxidant properties, various phytochemicals are found to alleviate the symptoms of F- toxicity. Hence, we explore the protective effect of esculin (Esc), a coumarin glucoside on F--induced oxidative stress and cardiotoxicity in zebrafish larvae. The experimental groups consisted of NaF (50 ppm) and Esc (100 μM) groups treated alone and in combination with a control group for 6 h. The groups were maintained till 78 hpf after which the level of oxidants (ROS, LPO, and PCC) and antioxidants (GST, GSH, GPx, SOD, and CAT) were assessed. The results revealed that Esc pretreatment restored the depleted antioxidant markers and reduced the levels of oxidant in the Esc+NaF group, exhibiting its antioxidant potential. In addition, analyses of the heartbeat rate and hemoglobin integrity using o-Dianisidine staining were conducted in the control and experimental groups. Esc treatment prevents F- induced cardiac changes including tachycardia and altered blood flow. Further, the mRNA expression level of antioxidant genes (nrf2, gstp1, hmox1a, prdx1, and nqo1) and cardiac developmental genes (bmp2b, nkx2.5, myh6, and myl7) confirmed that Esc acts as a potent free radical scavenger and antioxidant defense enhancer, protecting zebrafish larvae from NaF-induced oxidative stress and heart dysfunction.

香豆素葡萄糖苷Esculin可预防氟化物诱导的斑马鱼幼体氧化应激和心脏毒性
氟化物(F-)是一种遍布全球的主要地下水污染物。如果浓度过高,氟会对生物造成危害。氟暴露与细胞氧化还原失衡有关,会导致氧化应激介导的病变,包括心脏功能障碍。由于其强大的抗氧化特性,各种植物化学物质被发现可以减轻 F- 毒性的症状。因此,我们探讨了香豆素葡萄糖苷(esc)对斑马鱼幼体由芴诱导的氧化应激和心脏毒性的保护作用。实验组包括 NaF(50 ppm)组和 Esc(100 μM)组,分别单独或与对照组联合处理 6 小时。实验组维持至 78 hpf,之后评估氧化剂(ROS、LPO 和 PCC)和抗氧化剂(GST、GSH、GPx、SOD 和 CAT)的水平。结果表明,\预处理可恢复\+NaF组已耗竭的抗氧化剂指标,并降低氧化剂水平,显示了其抗氧化潜力。此外,还利用邻苯二甲酸二异噻啶染色法对对照组和实验组的心跳率和血红蛋白完整性进行了分析。Esc 处理可防止 F- 引起的心脏变化,包括心动过速和血流改变。此外,抗氧化基因(nrf2、gstp1、hmox1a、prdx1和nqo1)和心脏发育基因(bmp2b、nkx2.5、myh6和myl7)的mRNA表达水平证实,\是一种有效的自由基清除剂和抗氧化防御增强剂,可保护斑马鱼幼体免受NaF诱导的氧化应激和心脏功能障碍的影响。
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来源期刊
Environmental Toxicology
Environmental Toxicology 环境科学-毒理学
CiteScore
7.10
自引率
8.90%
发文量
261
审稿时长
4.5 months
期刊介绍: The journal publishes in the areas of toxicity and toxicology of environmental pollutants in air, dust, sediment, soil and water, and natural toxins in the environment.Of particular interest are: Toxic or biologically disruptive impacts of anthropogenic chemicals such as pharmaceuticals, industrial organics, agricultural chemicals, and by-products such as chlorinated compounds from water disinfection and waste incineration; Natural toxins and their impacts; Biotransformation and metabolism of toxigenic compounds, food chains for toxin accumulation or biodegradation; Assays of toxicity, endocrine disruption, mutagenicity, carcinogenicity, ecosystem impact and health hazard; Environmental and public health risk assessment, environmental guidelines, environmental policy for toxicants.
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