PA5402-5407 of Pseudomonas aeruginosa Regulate the Expression of the Aa3-Type Oxidases and Their Growth Under Carbon Starvation and High-Density Conditions.

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Haiyu Sun, Shenping Wang, Shuheng Du, Na Wang, Runqing Shi, Kairui Zhao, Chenghao Huang, Yaodong Chen
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引用次数: 0

Abstract

Our previous studies identified PA5407 in Pseudomonas aeruginosa as a new regulatory protein for bacterial division and named it ZapAL. This protein enhances the assembly of the key bacterial division protein FtsZ and participates in the assembly of the bacterial Z-ring, but its physiological function is not clear. ZapAL is in the same gene cluster as PA5402-5406, and in this study, we found that these genes are involved in the regulation of bacterial growth under nutrient deficiency and high-density conditions. The expression of Aa3 oxidase increases significantly at the end of the stationary phase of bacterial growth under aerobic conditions, and appropriately accelerate energy intake to adapt to the adversity. In our study, we found that the knockout of PA5402-5407 in P. aeruginosa promotes the further expression of Aa3 oxidase and its expression increased more and faster than the wild type, especially under carbon starvation and high-density conditions. This results in a larger bacteria population, but the average length of the bacteria is abnormally reduced. In summary, our study found that the P. aeruginosa gene cluster PA5402-5407 regulated the expression of Aa3 oxidase in the late stationary phase, and these genes balance energy intake, growth and division of bacteria under adverse conditions.

铜绿假单胞菌 PA5402-5407 在碳饥饿和高密度条件下调控 Aa3 型氧化酶的表达及其生长
我们之前的研究发现铜绿假单胞菌中的 PA5407 是一种新的细菌分裂调控蛋白,并将其命名为 ZapAL。该蛋白能增强关键细菌分裂蛋白 FtsZ 的组装,并参与细菌 Z 环的组装,但其生理功能尚不清楚。ZapAL与PA5402-5406同属一个基因簇,本研究发现这些基因参与了营养缺乏和高密度条件下细菌生长的调控。在有氧条件下细菌生长的静止期末期,Aa3 氧化酶的表达量明显增加,并适当加快能量摄入以适应逆境。在我们的研究中,我们发现铜绿微囊藻中 PA5402-5407 的敲除促进了 Aa3 氧化酶的进一步表达,特别是在碳饥饿和高密度条件下,其表达量的增加比野生型更多、更快。这导致细菌数量增加,但细菌的平均长度却异常减少。综上所述,我们的研究发现铜绿微囊藻基因簇 PA5402-5407 在静止后期调控 Aa3 氧化酶的表达,这些基因在不利条件下平衡了细菌的能量摄入、生长和分裂。
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来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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