Effects of ploidy level on leaf morphology, stomata, and anatomical structure of Hibiscus syriacus L.

Abstract

Background: Hibiscus syriacus L. is a deciduous shrub with a strong environmental resistance and wide application prospects. The genetic background and ploidy levels of Hibiscus cultivars are complex, and polyploid breeding has long been an important method for developing new Hibiscus cultivars. However, the relationship of ploidy levels with leaf morphology, stomatal characteristics, and leaf anatomy remains unclear.

Results: This study analyzed three ploidy levels (triploid, tetraploid, and hexaploid) of Hibiscus syriacus. Flow cytometry confirmed the ploidy levels, and morphological traits were evaluated. Leaf length, leaf width, and petiole length decreased with increasing ploidy. Stomatal length, stomatal width, guard cell length, and guard cell width increased and stomatal number and density decreased with increasing ploidy. The hexaploids exhibited the highest midrib diameter and palisade tissue thickness values. Correlation analyses revealed that stomatal morphology served as a reliable marker for determining ploidy levels.

Conclusion: This study highlights the impact of varying ploidy levels on the leaf and stomatal morphologies and leaf anatomy of Hibiscus syriacus. These findings can provide theoretical guidance for improving Hibiscus cultivars in terms of stress resistance, adaptability, and ornamental traits, and for developing new cultivars with enhanced characteristics. Future research should focus on utilizing these morphological markers to optimize breeding strategies for Hibiscus cultivars.