Endothelial activation, Cell-Cell Interactions, and Inflammatory Pathways in Postoperative Atrial Fibrillation Following Cardiac Surgery.

IF 4.1 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Raquel López-Gálvez, José Miguel Rivera-Caravaca, Darío Mandaglio-Collados, Antonio J Ruiz-Alcaraz, Álvaro Lahoz-Tornos, Diana Hernández-Romero, Esteban Orenes-Piñero, María Pilar Ramos-Bratos, Carlos M Martínez, Marina Carpes, José María Arribas-Leal, Sergio Cánovas, Gregory Y H Lip, Francisco Marín
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引用次数: 0

Abstract

Background: Postoperative atrial fibrillation (POAF) is common after cardiac surgery and related to endothelial activation and systemic inflammation. Herein, we investigate the pathophysiological mechanisms of AF through endothelial activation and cell-cell interactions related to the development of POAF.

Methods: Patients without previous AF undergoing cardiac surgery were studied. Permanent AF patients were included as positive controls. Interleukin (IL)-6, Von Willebrand factor (vWF), N-terminal pro-brain natriuretic peptide (NT-proBNP) and high sensitivity troponin T (hsTnT) were evaluated by electrochemiluminescence. Vascular cell adhesion molecule-1 (VCAM-1) and human Growth Differentiation Factor 15 (GDF-15) was assessed by ELISA. Connexins (Cxs) 40 and 43 were measured by tissue immunolabelling, and apoptosis by TUNEL assay.

Results: We included 117 patients (median age 67: 27.8% female): 17 with permanent AF; 27 with POAF and 73 with non- AF. Patients with permanent AF and POAF had higher levels of NT-proBNP, hs-TnT, apoptotic nuclei and decrease Cx43 expression, compared to non-AF patients (all p-value <0.05). VCAM-1 and GDF-15 were significantly higher in permanent AF vs. non-AF (p=0.013 and p=0.035).

Conclusions: Greater endothelial activation and inflammation in AF patients compared to those without AF was found. The proinflammatory state in AF patients, in addition to the lower expression of Cx43, seems to be associated with atrial remodeling processes occurring in AF.

心脏手术后心房颤动的内皮活化、细胞间相互作用和炎症途径
背景:术后心房颤动(POAF)是心脏手术后的常见病,与内皮激活和全身炎症有关。在此,我们研究了心房颤动通过内皮活化和细胞-细胞间相互作用与 POAF 发生相关的病理生理机制:方法:研究对象为接受心脏手术的既往无房颤患者。方法:研究对象为接受心脏手术的无房颤患者,永久性房颤患者作为阳性对照。白细胞介素(IL)-6、冯-威廉因子(vWF)、N末端前脑钠尿肽(NT-proBNP)和高敏肌钙蛋白T(hsTnT)通过电化学发光法进行评估。血管细胞粘附分子-1(VCAM-1)和人生长分化因子 15(GDF-15)通过 ELISA 进行评估。通过组织免疫标记法测定连接蛋白(Cxs)40和43,通过TUNEL法测定细胞凋亡:我们共纳入了 117 名患者(中位年龄 67 岁:27.8% 为女性):其中 17 人患有永久性房颤;27 人患有 POAF;73 人患有非房颤。与非房颤患者相比,永久性房颤和 POAF 患者的 NT-proBNP、hs-TnT、细胞核凋亡水平较高,Cx43 表达减少(均为 p 值 结论:与非房颤患者相比,永久性房颤和 POAF 患者的 NT-proBNP、hs-TnT、细胞核凋亡水平较高,Cx43 表达减少:与非房颤患者相比,房颤患者的内皮活化和炎症反应更为严重。心房颤动患者的促炎症状态以及较低的 Cx43 表达似乎与心房颤动中发生的心房重塑过程有关。
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来源期刊
Biomedical Journal
Biomedical Journal Medicine-General Medicine
CiteScore
11.60
自引率
1.80%
发文量
128
审稿时长
42 days
期刊介绍: Biomedical Journal publishes 6 peer-reviewed issues per year in all fields of clinical and biomedical sciences for an internationally diverse authorship. Unlike most open access journals, which are free to readers but not authors, Biomedical Journal does not charge for subscription, submission, processing or publication of manuscripts, nor for color reproduction of photographs. Clinical studies, accounts of clinical trials, biomarker studies, and characterization of human pathogens are within the scope of the journal, as well as basic studies in model species such as Escherichia coli, Caenorhabditis elegans, Drosophila melanogaster, and Mus musculus revealing the function of molecules, cells, and tissues relevant for human health. However, articles on other species can be published if they contribute to our understanding of basic mechanisms of biology. A highly-cited international editorial board assures timely publication of manuscripts. Reviews on recent progress in biomedical sciences are commissioned by the editors.
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