Insights Into the Composition and Antibacterial Activity of Different Parts of Alpinia katsumadai Hayata Based on HS-SPME-GC-MS and HS-GC-IMS

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2024-11-28 DOI:10.1002/rcm.9949

Jiaxuan Chen, Xue Chen, Yang Zhang, Jingyi Xie, Xuejuan Li, Xuehao Sun, Jiaxin Yin, Songtao Bie, Xinbo Song, Heshui Yu, Zheng Li

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引用次数: 0

Abstract

Rationale

The volatile organic compounds (VOCs) of Alpinia katsumadai Hayata (AKH) play a key role in determining its effects such as organoleptic properties, medicinal properties, and consumer preferences. The nonmedicinal parts (roots, fibrous roots, stems, leaves, and shells) in AKH are also rich in VOCs and different degrees of antibacterial activity. Therefore, it is important to comprehensively characterize the VOCs in different parts of AKH and learn about their potential antimicrobial abilities.

Methods

In this study, headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) and headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS) were used to comprehensively investigate the compositional characteristics and differences of roots, fibrous roots, stems, leaves, shells, and seeds in AKH. Multivariate statistical analysis was used to determine the differential VOCs in different parts of AKH. The inhibition zone diameter method was used to compare the antibacterial ability of different parts.

Results

HS-GC-IMS and HS-SPME-GC-MS analyses identified 67 and 125 VOCs, respectively. Multivariate statistical analysis showed the differences in the six parts is obvious. Compounds such as (E)-2-heptenal and thymol were selected as potential VOCs to distinguish six sites of AKH. In general, the inhibition effect of the volatile oils from shell and root on the two colonies was better, and the volatile oils of the four parts of AKH have better inhibitory effect on Staphylococcus aureus.

Conclusion

This research conducted a comprehensive analysis of the fundamental volatile components in AKH. It subsequently elucidated the distinctions and specificities in different parts. The antibacterial effect of volatile oil in different parts was further compared, which was conducive to finding alternative parts for seeds in some active aspects, and the utilization of AKH was more sufficient.

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基于 HS-SPME-GC-MS 和 HS-GC-IMS 对 Alpinia katsumadai Hayata 不同部位的成分和抗菌活性的深入研究

理由：Alpinia katsumadai Hayata（AKH）的挥发性有机化合物（VOCs）在决定其感官特性、药用特性和消费者偏好等效果方面起着关键作用。AKH 中的非药用部分（根、须根、茎、叶和壳）也含有丰富的挥发性有机化合物和不同程度的抗菌活性。因此，全面描述 AKH 不同部位的挥发性有机化合物的特征并了解其潜在的抗菌能力非常重要：本研究采用顶空气相色谱-离子迁移谱法（HS-GC-IMS）和顶空固相微萃取气相色谱-质谱法（HS-SPME-GC-MS）全面研究了 AKH 中根、须根、茎、叶、壳和种子的成分特征及差异。采用多元统计分析确定了 AKH 不同部位挥发性有机化合物的差异。采用抑菌区直径法比较不同部位的抗菌能力：结果：HS-GC-IMS 和 HS-SPME-GC-MS 分析分别鉴定出 67 种和 125 种挥发性有机化合物。多变量统计分析显示，六种成分的抗菌能力差异明显。(E)-2-庚烯醛和百里酚等化合物被选为潜在的挥发性有机化合物，以区分 AKH 的六个部位。总的来说，壳和根的挥发油对两种菌落的抑制效果较好，AKH 四个部位的挥发油对金黄色葡萄球菌有较好的抑制效果：本研究对 AKH 中的基本挥发性成分进行了全面分析。结论：本研究对 AKH 中的基本挥发性成分进行了全面分析，随后阐明了不同部位的区别和特异性。进一步比较了不同部位挥发油的抗菌效果，有利于在某些活性方面找到种子的替代部位，更充分地利用 AKH。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.