Biopolymer-based oral films integrated with probiotic active compounds for improved health applications

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Sinem Tunçer Çağlayan
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Abstract

Orally dissolving films (ODFs) have emerged as a versatile platform that combines convenience, efficacy, and patient compliance. In this study, the cell-free supernatant of the oral probiotic Streptococcus salivarius M18 was incorporated into various biopolymer-based ODF formulations, evaluated for demolding, fragility, and flexibility. The combination of carboxymethyl cellulose, sodium alginate, and glycerol successfully formed stable films. The films were characterized by weight, thickness, pH, and disintegration times. Fourier-transform infrared spectroscopy (FTIR) was used to analyze ODF content and release profiles in simulated saliva. Unique absorption peaks in the cell-free product-incorporated ODF samples confirmed the integration of bacterial proteins, lipids, and nucleic acids into the ODF matrix. The biological activity of the ODF carrying M18 bioactive products was assessed by its inhibitory effect on the growth of Streptococcus mutans, a pathogen linked to dental plaque and cavities. Additionally, the anti-proliferative effect on cancer epithelial cells was demonstrated. This study show that probiotic products can be integrated into bio-based thin films without losing activity, making this delivery platform promising for local and potentially systemic effects.

Abstract Image

基于生物聚合物的口腔薄膜集成了益生菌活性化合物,可用于改善健康状况
口腔溶解膜(ODF)是一种集方便性、有效性和患者依从性于一体的多功能平台。在这项研究中,将口服益生菌唾液链球菌 M18 的无细胞上清液加入各种基于生物聚合物的 ODF 配方中,并对其脱模性、脆性和柔韧性进行了评估。羧甲基纤维素、海藻酸钠和甘油的组合成功地形成了稳定的薄膜。对薄膜的重量、厚度、pH 值和崩解时间进行了表征。傅立叶变换红外光谱(FTIR)用于分析 ODF 的含量和在模拟唾液中的释放曲线。无细胞产品融入 ODF 样品中的独特吸收峰证实了细菌蛋白质、脂类和核酸融入了 ODF 基质。携带 M18 生物活性产品的 ODF 的生物活性通过其对变异链球菌生长的抑制作用进行了评估,变异链球菌是一种与牙菌斑和龋齿有关的病原体。此外,还证明了其对癌症上皮细胞的抗增殖作用。这项研究表明,益生菌产品可以融入生物基薄膜而不会失去活性,因此这种递送平台很有希望产生局部和潜在的全身效应。
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来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
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