Cerasicoccus fimbriatus sp. nov., isolated from the mid-ridge of the Southwest Indian Ocean

Abstract

A Gram-stain-negative bacterium, designated as strain TK19100^T, was isolated from the mid-ridge of the Southwest Indian Ocean. Cells of strain TK19100^T were strictly aerobic, non-motile and short-rod shaped with fimbriae-like structures around the cell surface. Growth occurred at 15–40 °C, at pH 6.0–9.0 and with 1–10% (w/v) NaCl. Strain TK19100^T shared the highest 16S rRNA gene sequence similarity with Cerasicoccus frondis NBRC 105381^T of 97.74%, followed by Cerasicoccus arenae KCTC 12870^T of 97.69% and Cerasicoccus maritimus NBRC 105382^T of 97.40%. The phylogenetic tree based on both 16S rRNA genes and 92 core genes placed strain TK19100^T in a new linage within the genus Cerasicoccus. The genome size of strain TK19100^T was 5.07 Mb with the DNA G + C content 56.1%. The average nucleotide identity value and the digital DNA-DNA hybridization value of strain TK19100^T compared with the closest related species, Cerasicoccus frondis, were 78.39% and 23.70%, respectively. Strain TK19100^T encoded a previously unreported combination of GH16 and Carbohydrate Binding Module 96. The major fatty acids of strain TK19100^T were C_14:0 and C_18:1 ω9c. Menaquinone-7 was the sole respiratory quinone. The phenotypic and genotypic characterization analysis indicate that strain TK19100^T represents a novel species affiliated to the genus Cerasicoccus, for which the name Cerasicoccus fimbriatus sp. nov. is proposed. The type strain is TK19100^T (= CGMCC 1.18957^T = NBRC 116189^T).