Valorization of Pineapple Waste for Extraction and Purification of Bromelain Enzyme

Varunraj Gharge, Sahil Ghutake and Hitesh Pawar*, 
{"title":"Valorization of Pineapple Waste for Extraction and Purification of Bromelain Enzyme","authors":"Varunraj Gharge,&nbsp;Sahil Ghutake and Hitesh Pawar*,&nbsp;","doi":"10.1021/acssusresmgt.4c0028310.1021/acssusresmgt.4c00283","DOIUrl":null,"url":null,"abstract":"<p >Bromelain is a potential proteolytic enzyme present in various parts of pineapple, having industrial importance with a wide range of applications. Present study focused on extraction, isolation, and purification of bromelain from pineapple peels waste as a feedstock. The proximate analysis of pineapple peel waste depicts the presence of about 7% protein in pineapple peel waste. Thus, several commercially available buffers were explored for the extraction of bromelain from pineapple peel. Of the tested buffers, sodium phosphate buffer showed the highest bromelain activity (0.75 CDU/mL) and protein concentration (0.160 mg/mL). The process intensification study is conducted to achieve maximum bromelain activity and maximum protein concentration. The optimized process provided maximum bromelain activity as 1.24 CDU/mL and maximum protein concentration of 0.130 mg/mL. The combination of ultrafiltration and adsorptive chromatographic purification was used to concentrate bromelain and to remove color components, respectively. It provides an increase in bromelain activity by 2.24-fold and protein concentration by 1.37 folds. Thus, the present study provides an efficient strategy for valorization of pineapple peel waste through extraction and purification of bromelain as a potential product.</p>","PeriodicalId":100015,"journal":{"name":"ACS Sustainable Resource Management","volume":"1 11","pages":"2439–2451 2439–2451"},"PeriodicalIF":0.0000,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Sustainable Resource Management","FirstCategoryId":"1085","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acssusresmgt.4c00283","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Bromelain is a potential proteolytic enzyme present in various parts of pineapple, having industrial importance with a wide range of applications. Present study focused on extraction, isolation, and purification of bromelain from pineapple peels waste as a feedstock. The proximate analysis of pineapple peel waste depicts the presence of about 7% protein in pineapple peel waste. Thus, several commercially available buffers were explored for the extraction of bromelain from pineapple peel. Of the tested buffers, sodium phosphate buffer showed the highest bromelain activity (0.75 CDU/mL) and protein concentration (0.160 mg/mL). The process intensification study is conducted to achieve maximum bromelain activity and maximum protein concentration. The optimized process provided maximum bromelain activity as 1.24 CDU/mL and maximum protein concentration of 0.130 mg/mL. The combination of ultrafiltration and adsorptive chromatographic purification was used to concentrate bromelain and to remove color components, respectively. It provides an increase in bromelain activity by 2.24-fold and protein concentration by 1.37 folds. Thus, the present study provides an efficient strategy for valorization of pineapple peel waste through extraction and purification of bromelain as a potential product.

Abstract Image

菠萝废料在提取和纯化菠萝蛋白酶中的应用
菠萝蛋白酶是一种潜在的蛋白水解酶,存在于菠萝的各个部位,具有广泛的工业用途。本研究的重点是从作为原料的菠萝皮废料中提取、分离和纯化菠萝蛋白酶。菠萝皮废料的近似分析表明,菠萝皮废料中含有约 7% 的蛋白质。因此,我们研究了几种市售缓冲液,用于从菠萝皮中提取菠萝蛋白酶。在测试的缓冲液中,磷酸钠缓冲液显示出最高的菠萝蛋白酶活性(0.75 CDU/mL)和蛋白质浓度(0.160 mg/mL)。为了获得最高的菠萝蛋白酶活性和最高的蛋白质浓度,进行了工艺强化研究。优化后的工艺可获得最高的菠萝蛋白酶活性(1.24 CDU/mL)和最高的蛋白质浓度(0.130 mg/mL)。超滤和吸附色谱纯化相结合,分别用于浓缩菠萝蛋白酶和去除色素成分。它使菠萝蛋白酶活性提高了 2.24 倍,蛋白质浓度提高了 1.37 倍。因此,本研究通过提取和纯化菠萝蛋白酶作为一种潜在的产品,为菠萝皮废弃物的增值提供了一种有效的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信