Pyrene functionalized organic cation receptor-based “turn-on” fluorescence approach for monitoring of chlorpyrifos in food, soil, and water samples

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2024-11-28 DOI:10.1016/j.aca.2024.343488

Manish Kumar , Aman Dhiman , Gagandeep Singh , Navneet Kaur , Narinder Singh

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引用次数: 0

Abstract

Background

The unregulated use of pesticides by farmers, for crop productivity results in widespread contamination of organophosphates in real environmental samples, which is a growing societal concern about their potential health effects. The conventional approaches for the monitoring these organophosphate-based pesticides which include immunoassays, electrochemical methods, immunosensors, various chromatography techniques, along with some spectroscopic methods, are either costly, sophisticated, or involves the use of different metal complexes. Therefore, there is an urgent need for sensitive, quick, and easy-to-use detection techniques for the screening of widely used organophosphate-based pesticides.

Results

Herein, we demonstrates the metal-free detection of CPF pesticide in aqueous medium, based on the organic nanoparticles of benzimidazole-based cationic receptor (R1-ONPs), and thoroughly analyzed using advanced techniques such as AFM, FESEM, and DLS etc. The photophysical investigations revealed that developed R1-ONPs exhibited high selectivity towards chlorpyrifos with an enhancement in fluorescence emission. Further, the observed pyrene excimer-based "turn-on" fluorescence mechanism, and the interaction between developed sensor and chlorpyrifos has been validated utilizing ¹H, and ³¹P NMR spectroscopy. The developed sensor can effectively quantify chlorpyrifos up to a detection limit of 18.9 nM (3σ method) with a range of 0–120 μM as well as below the cutoff limit set by FAO. Moreover, the real-time application of developed sensor (R1-ONPs) was evaluated to monitor chlorpyrifos in spiked food, water, and soil samples with good (%) recovery.

Significance

The development of metal-free, pyrene-excimer-based “Turn-On” fluorescent sensor offers a novel, eco-friendly strategy for the detection of chlorpyrifos in aqueous medium. Additionally, its ability to quantify the chlorpyrifos at levels as low as those set by FAO makes it more efficient tool for monitoring the environmental toxicity ensuring better protection for human, and animal health.

Abstract Image

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基于芘功能化有机受体的 "开启式 "荧光方法，用于监测食品、土壤和水样中的毒死蜱

背景农民为了提高作物产量而无节制地使用杀虫剂，导致实际环境样本中广泛存在有机磷污染，这引起了社会对其潜在健康影响的日益关注。监测这些有机磷农药的传统方法包括免疫测定、电化学方法、免疫传感器、各种色谱技术以及一些光谱方法，但这些方法要么成本高昂、技术复杂，要么需要使用不同的金属络合物。因此，迫切需要灵敏、快速、易用的检测技术来筛查广泛使用的有机磷农药。结果在本文中，我们展示了基于苯并咪唑阳离子受体有机纳米粒子（R1-ONPs）的水介质中 CPF 农药的无金属检测，并使用原子力显微镜（AFM）、有限元电子显微镜（FESEM）和 DLS 等先进技术进行了深入分析。光物理研究表明，所开发的 R1-ONPs 对毒死蜱具有高选择性，并能增强荧光发射。此外，利用 1H 和 31P-NMR 光谱验证了所观察到的基于芘准分子的 "开启 "荧光机制，以及所开发传感器与毒死蜱之间的相互作用。所开发的传感器能有效地定量毒死蜱，检测限为 18.9 nM（3σ 法），检测范围为 0-120 μM，低于联合国粮农组织规定的临界值。此外，对所开发传感器（R1-ONPs）的实时应用进行了评估，以监测添加了毒死蜱的食品、水和土壤样品，回收率良好（%）。重要意义开发无金属、基于芘-拮抗剂的 "Turn-On "荧光传感器为检测水介质中的毒死蜱提供了一种新颖、环保的策略。此外，它还能在联合国粮农组织规定的低浓度水平上对毒死蜱进行定量检测，从而使其成为监测环境毒性的更有效工具，确保更好地保护人类和动物的健康。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.