Mating Behaviour Influences the Direction and Geographic Extent of Introgression in New Zealand Fishing Spiders (Dolomedes).

Abstract

Introgression is a highly influential process in evolution, where genes flow between species that are not fully reproductively isolated. Studies on introgression often focus on describing gene transfer and environmental changes that facilitate the meeting of species. However, the impact of mating systems and behaviour that facilitate gene transfer is less well-known. Dolomedes aquaticus and D. minor are sister species of fishing spiders undergoing one-way, geographically limited mitochondrial introgression, making them an excellent case study for the factors that promote and limit introgression. We used a combination of field observations and crossing experiments to systematically investigate potential pre-fertilisation barriers that could limit introgression and explain the one-way and geographic barriers to introgression in these species. We found that habitat overlap and timing of reproductive maturity were not likely to be important limiting factors to introgression. However, behaviour was an important factor, with male mate choice being implicated in the geographic limitation, and female mate choice being implicated in the one-way limitation. Our results show the importance of using behavioural approaches in the investigation of introgression.