Pathological Study of Demyelination with Cellular Reactions in the Cerebellum of Dogs Infected with Canine Distemper Virus.

IF 3.8 3区 医学 Q2 VIROLOGY
Viruses-Basel Pub Date : 2024-10-31 DOI:10.3390/v16111719
José Manuel Verdes, Camila Larrañaga, Guillermo Godiño, Belén Varela, Victoria Yozzi, Victoria Iribarnegaray, Luis Delucchi, Kanji Yamasaki
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Abstract

The purpose of this study was to examine the relationship between demyelination and cellular reactions in the cerebellum of Canine Distemper Virus (CDV)-infected dogs. We subdivided the disease staging by adding the degree of demyelination determined by Luxol Fast Blue staining to the previously reported disease staging from the acute stage to the chronic stage, and investigated the relationship between demyelination in the cerebellum and the number and histological changes in astroglia, microglia, and Purkinje cells in each stage. Reactions of astrocytes and microglia were observed at an early stage when demyelination was not evident. Changes progressed with demyelination. Demyelination initially began in the medulla adjoining the fourth ventricle and gradually spread to the entire cerebellum, including the lobes. CDV immune-positive granules were seen from the early stage, and inclusion bodies also appeared at the same time. CDV immune-positive reaction and inclusion bodies were observed in astrocytes, microglia, neurons, ependymal cells, and even leptomeningeal mononuclear cells. On the other hand, infiltration of CDV-immunoreactive particles from the pia mater to the gray matter and further into the white matter through the granular layer was observed from an early stage. Purkinje cells decreased from the intermediate stage, and a decrease in cells in the granular layer was also observed. There was no clear association between age and each stage, and the stages did not progress with age.

犬瘟热病毒感染犬小脑脱髓鞘及细胞反应的病理学研究
本研究旨在探讨犬瘟热病毒(CDV)感染犬小脑脱髓鞘与细胞反应之间的关系。我们在之前报道的疾病分期基础上,根据卢克索快速蓝染色法确定的脱髓鞘程度,将疾病分期从急性期细分为慢性期,并研究了小脑脱髓鞘与各期星形胶质细胞、小胶质细胞和浦肯野细胞的数量和组织学变化之间的关系。在脱髓鞘不明显的早期阶段,就观察到了星形胶质细胞和小胶质细胞的反应。随着脱髓鞘的发展,变化也在不断加剧。脱髓鞘最初开始于与第四脑室相邻的髓质,然后逐渐扩散到整个小脑,包括小脑叶。CDV 免疫阳性颗粒在早期就已出现,包涵体也同时出现。在星形胶质细胞、小胶质细胞、神经元、上皮细胞,甚至脑膜单核细胞中均可观察到 CDV 免疫阳性反应和包涵体。另一方面,从早期阶段就观察到 CDV 免疫反应颗粒从桥脑浸润到灰质,并通过颗粒层进一步浸润到白质。浦肯野细胞从中期开始减少,颗粒层细胞也出现减少。年龄与各阶段之间没有明显的联系,各阶段也没有随着年龄的增长而进展。
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来源期刊
Viruses-Basel
Viruses-Basel VIROLOGY-
CiteScore
7.30
自引率
12.80%
发文量
2445
审稿时长
1 months
期刊介绍: Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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