Longitudinal 1H NMR-Based Metabolomics in Saliva Unveils Signatures of Transition from Acute to Post-Acute Phase of SARS-CoV-2 Infection.

IF 3.8 3区 医学 Q2 VIROLOGY
Viruses-Basel Pub Date : 2024-11-13 DOI:10.3390/v16111769
Luiza Tomé Mendes, Marcos C Gama-Almeida, Desirée Lopes Reis, Ana Carolina Pires E Silva, Rômulo Leão Silva Neris, Rafael Mello Galliez, Terezinha Marta Pereira Pinto Castiñeiras, On Behalf Of The Ufrj Covid-Working Group, Christian Ludwig, Ana Paula Valente, Gilson Costa Dos Santos Junior, Tatiana El-Bacha, Iranaia Assunção-Miranda
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Abstract

COVID-19 can range from a mild to severe acute respiratory syndrome and also could result in multisystemic damage. Additionally, many people develop post-acute symptoms associated with immune and metabolic disturbances in response to viral infection, requiring longitudinal and multisystem studies to understand the complexity of COVID-19 pathophysiology. Here, we conducted a 1H Nuclear Magnetic Resonance metabolomics in saliva of symptomatic subjects presenting mild and moderate respiratory symptoms to investigate prospective changes in the metabolism induced after acute-phase SARS-CoV-2 infection. Saliva from 119 donors presenting non-COVID and COVID-19 respiratory symptoms were evaluated in the acute phase (T1) and the post-acute phase (T2). We found two clusters of metabolite fluctuation in the COVID-19 group. Cluster 1, metabolites such as glucose, (CH3)3 choline-related metabolites, 2-hydroxybutyrate, BCAA, and taurine increased in T2 relative to T1, and in cluster 2, acetate, creatine/creatinine, phenylalanine, histidine, and lysine decreased in T2 relative to T1. Metabolic fluctuations in the COVID-19 group were associated with overweight/obesity, vaccination status, higher viral load, and viral clearance of the respiratory tract. Our data unveil metabolic signatures associated with the transition to the post-acute phase of SARS-CoV-2 infection that may reflect tissue damage, inflammatory process, and activation of tissue repair cascade. Thus, they contribute to describing alterations in host metabolism that may be associated with prolonged symptoms of COVID-19.

基于纵向 1H NMR 的唾液代谢组学揭示了 SARS-CoV-2 感染从急性期向急性期后过渡的特征。
COVID-19 可导致轻度到重度的急性呼吸系统综合征,还可能造成多系统损害。此外,许多人在病毒感染后会出现与免疫和代谢紊乱相关的急性期后症状,因此需要进行纵向和多系统研究,以了解 COVID-19 病理生理学的复杂性。在此,我们对出现轻度和中度呼吸道症状的受试者唾液进行了 1H 核磁共振代谢组学研究,以探讨急性期 SARS-CoV-2 感染后诱发的代谢前瞻性变化。我们对 119 名出现非 COVID 和 COVID-19 呼吸道症状的供体在急性期(T1)和急性期后(T2)的唾液进行了评估。我们发现 COVID-19 组有两个代谢物波动群。群组 1 中,葡萄糖、(CH3)3 胆碱相关代谢物、2-羟基丁酸、BCAA 和牛磺酸等代谢物在 T2 中相对于 T1 有所增加;群组 2 中,醋酸、肌酸/肌酐、苯丙氨酸、组氨酸和赖氨酸在 T2 中相对于 T1 有所减少。COVID-19 组的代谢波动与超重/肥胖、疫苗接种状况、较高的病毒载量和呼吸道病毒清除率有关。我们的数据揭示了与 SARS-CoV-2 感染后急性期过渡相关的代谢特征,这些特征可能反映了组织损伤、炎症过程和组织修复级联的激活。因此,这些数据有助于描述宿主新陈代谢的改变,而宿主新陈代谢的改变可能与 COVID-19 的症状延长有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Viruses-Basel
Viruses-Basel VIROLOGY-
CiteScore
7.30
自引率
12.80%
发文量
2445
审稿时长
1 months
期刊介绍: Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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