RNA-Seq Reveals Transcriptome Changes Following Zika Virus Infection in Fetal Brains in c-Flip Knockdown Mice.

IF 3.8 3区 医学 Q2 VIROLOGY
Viruses-Basel Pub Date : 2024-10-31 DOI:10.3390/v16111712
Ting Xie, Qiqi Chen, Nina Li, Shengze Zhang, Lin Zhu, Shaohui Bai, Haolu Zha, Weijian Tian, Chuming Luo, Nan Wu, Xuan Zou, Shisong Fang, Yuelong Shu, Jianhui Yuan, Ying Jiang, Huanle Luo
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引用次数: 0

Abstract

The FADD-like interleukin-1β converting enzyme (FLICE)-inhibitory protein (c-FLIP) plays a crucial role in various biological processes, including apoptosis and inflammation. However, the complete transcriptional profile altered by the c-FLIP is not fully understood. Furthermore, the impact of the c-FLIP deficiency on the transcriptome during a Zika virus (ZIKV) infection, which induces apoptosis and inflammation in the central nervous system (CNS), has not yet been elucidated. In this study, we compared transcriptome profiles between wild-type (WT) and the c-Flip heterozygous knockout mice (c-Flip+/-) fetal heads at embryonic day 13.5 from control and PBS-infected WT dams mated with c-Flip+/- sires. In the non-infected group, we observed differentially expressed genes (DEGs) mainly involved in embryonic development and neuron development. However, the ZIKV infection significantly altered the transcriptional profile between WT and the c-Flip+/- fetal heads. DEGs in pattern recognition receptor (PRR)-related signaling pathways, such as the RIG-I-like receptor signaling pathway and Toll-like receptor signaling pathway, were enriched. Moreover, the DEGs were also enriched in T cells, indicating that the c-FLIP participates in both innate and adaptive immune responses upon viral infection. Furthermore, our observations indicate that DEGs are associated with sensory organ development and eye development, suggesting a potential role for the c-FLIP in ZIKV-induced organ development defects. Overall, we have provided a comprehensive transcriptional profile for the c-FLIP and its modulation during a ZIKV infection.

RNA-Seq揭示了c-Flip基因敲除小鼠胎儿脑部感染寨卡病毒后转录组的变化。
FADD 样白细胞介素-1β转换酶(FLICE)抑制蛋白(c-FLIP)在包括细胞凋亡和炎症在内的各种生物过程中发挥着至关重要的作用。然而,c-FLIP 所改变的完整转录谱尚未完全清楚。此外,寨卡病毒(ZIKV)感染会诱导中枢神经系统(CNS)中的细胞凋亡和炎症,c-FLIP 的缺乏对中枢神经系统(CNS)转录组的影响尚未阐明。在这项研究中,我们比较了野生型小鼠(WT)和c-Flip杂合基因敲除小鼠(c-Flip+/-)在胚胎13.5天时的胎头转录组图谱,这些胎头来自对照组和PBS感染的WT母鼠与c-Flip+/-母鼠交配。在未感染组,我们观察到主要涉及胚胎发育和神经元发育的差异表达基因(DEGs)。然而,ZIKV感染显著改变了WT和c-Flip+/-胎头之间的转录谱。与模式识别受体(PRR)相关的信号通路(如RIG-I样受体信号通路和Toll样受体信号通路)中的DEGs被富集。此外,这些 DEGs 在 T 细胞中也有富集,这表明 c-FLIP 在病毒感染时参与了先天性和适应性免疫反应。此外,我们的观察结果表明,DEGs 与感觉器官发育和眼球发育有关,这表明 c-FLIP 在 ZIKV 诱导的器官发育缺陷中可能发挥作用。总之,我们为 c-FLIP 及其在 ZIKV 感染期间的调控提供了全面的转录概况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Viruses-Basel
Viruses-Basel VIROLOGY-
CiteScore
7.30
自引率
12.80%
发文量
2445
审稿时长
1 months
期刊介绍: Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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