The neurotoxic effect of Naja nubiae (Serpentes: Elapidae) venom from Sudan.

IF 1.9 4区医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2024-11-27 DOI:10.1093/trstmh/trae116

Huda Khalid, Maowia M Mukhtar

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引用次数: 0

Abstract

Background: Neurotoxicity is a common feature of elapid snake envenomation. There are limited studies on the toxicity of Naja nubiae venom, the Nubian spitting cobra, from north-east Africa.

Methods: We used the chick biventer cervicis nerve-muscle preparation to demonstrate the neurotoxic effect of N. nubiae venom and to compare it with the potent neurotoxic cobra Naja melanoleuca venom. Venoms were separated by successive reverse-phase high-performance liquid chromatography (RP-HPLC) runs and the molecular mass of the neurotoxins was determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS).

Results: Both venoms caused time-dependent inhibition of nerve-mediated twitches with a t90 value of 22.2±1.9 min and 12.9±1.2 min for N. nubiae and N. melanoleuca venoms, respectively. Prior incubation of some commercial antivenom (EchiTab-Plus-ICP [Costa Rica], CSL, Parkville, Victoria, Australia) and snake venom antisera [India]) did not prevent the neurotoxic effect of N. nubiae venom. The chromatographic separation of N. nubiae and N. melanoleuca venoms followed by MALDI-TOF MS analysis revealed that short-chain α-neurotoxin accounted for 8.4% of N. nubiae and 14.8% of N. melanoleuca whole venoms.

Conclusions: N. nubiae venom, which was previously known as cytotoxic venom, exhibits considerable in vitro neurotoxic effects on chick nerve-muscle preparations that may have consequences for antivenom development in north-east Africa.

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苏丹 Naja nubiae（蛇类：Elapidae）毒液的神经毒性效应。

背景：神经毒性是伶蛇类中毒的常见特征。有关非洲东北部努比亚吐丝眼镜蛇 Naja nubiae 毒液毒性的研究十分有限：方法：我们使用小鸡双颈神经肌肉制剂来证明 N. nubiae 毒液的神经毒性作用，并将其与具有强烈神经毒性的眼镜蛇 Naja melanoleuca 毒液进行比较。毒液通过连续反相高效液相色谱法（RP-HPLC）分离，神经毒素的分子质量通过基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF MS）测定：结果：N. nubiae和N. melanoleuca两种毒液对神经介导的抽搐均有时间依赖性抑制作用，t90值分别为22.2±1.9 min和12.9±1.2 min。事先孵育一些商用抗蛇毒血清（EchiTab-Plus-ICP [哥斯达黎加]，CSL，Parkville，Victoria，澳大利亚）和蛇毒抗血清 [印度]）并不能阻止 N. nubiae 毒液的神经毒性作用。通过对努比亚蛇毒和黑斑鳢蛇毒进行色谱分离和 MALDI-TOF MS 分析，发现努比亚蛇毒和黑斑鳢蛇毒中的短链 α-神经毒素分别占整个蛇毒的 8.4% 和 14.8%：结论：N. nubiae毒液以前被称为细胞毒性毒液，在体外对小鸡神经肌肉制剂表现出相当大的神经毒性作用，这可能会对非洲东北部的抗蛇毒血清开发产生影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transactions of The Royal Society of Tropical Medicine and Hygiene 医学-公共卫生、环境卫生与职业卫生

CiteScore

4.00

自引率

9.10%

发文量

115

审稿时长

4-8 weeks

期刊介绍： Transactions of the Royal Society of Tropical Medicine and Hygiene publishes authoritative and impactful original, peer-reviewed articles and reviews on all aspects of tropical medicine.