Foodborne Pathogens Across Different Food Matrices in Sicily (Southern Italy).

IF 3.3 3区医学 Q2 MICROBIOLOGY

Pathogens Pub Date : 2024-11-14 DOI:10.3390/pathogens13110998

Valeria Blanda, Ilenia Giacchino, Valeria Vaglica, Vanessa Milioto, Sergio Migliore, Santina Di Bella, Francesca Gucciardi, Carmelo Bongiorno, Giuseppina Chiarenza, Cinzia Cardamone, Isabella Mancuso, Maria Luisa Scatassa, Vincenza Cannella, Annalisa Guercio, Giuseppa Purpari, Francesca Grippi

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Abstract

Foodborne diseases result from the consumption of foods contaminated with pathogens or their toxins and represent a serious public health problem worldwide. This study aimed to assess the presence of Rotavirus (RoV), Adenovirus (AdV), Norovirus (NoV), Hepatitis A and Hepatitis E viruses (HAV and HEV, respectively), Toxoplasma gondii, Coxiella burnetii and Leptospira spp. across various food matrices in Sicily. The analysis concerned 504 samples, including mussels, farmed meat, game meat, vegetables and bulk milk. Following appropriate pre-treatment, acid nucleic extraction was carried out and amplification of pathogen nucleic acids was carried out by molecular methods. The mussels tested positive for NoVs (3/51, 5.9%) and farm meat resulted positive for T. gondii (1/34, 2.9%). The game offal samples tested positive for HEV, which was detected in 17 out of 222 samples (7.7%), and T. gondii (18/318, 5.7%) and Leptospira spp. (2/318, 0.6%). The milk samples tested positive for C. burnetii (15/85, 17.6%), T. gondii (2/85, 2.4%) and Leptospira spp. (1/85, 1.2%). This study highlights the variability in the risk of contamination of different food matrices, confirming the importance of vigilance in the consumption of potentially contaminated food products.

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西西里岛（意大利南部）不同食物基质中的食源性病原体。

食源性疾病是由于食用了受病原体或其毒素污染的食物而引起的，是全球严重的公共卫生问题。这项研究旨在评估西西里岛各种食品基质中是否存在轮状病毒（RoV）、腺病毒（AdV）、诺罗病毒（NoV）、甲型肝炎病毒（HAV）和戊型肝炎病毒（HEV）、弓形虫、烧伤柯克氏菌和钩端螺旋体。分析涉及 504 个样本，包括贻贝、养殖肉类、野味肉类、蔬菜和散装牛奶。经过适当的预处理后，进行了酸核提取，并通过分子方法对病原体核酸进行了扩增。结果显示，贻贝样本中的 NoVs 呈阳性（3/51，5.9%），养殖肉类样本中的 T. gondii 呈阳性（1/34，2.9%）。野味内脏样本对高致病性乙型肝炎病毒（222 个样本中有 17 个样本检测出高致病性乙型肝炎病毒，占 7.7%）、淋球菌（18/318，占 5.7%）和钩端螺旋体（2/318，占 0.6%）检测呈阳性。牛奶样本对烧伤梭菌（15/85，17.6%）、淋球菌（2/85，2.4%）和钩端螺旋体（1/85，1.2%）检测呈阳性。这项研究强调了不同食物基质受污染风险的差异性，证实了在食用可能受污染的食品时保持警惕的重要性。

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来源期刊

Pathogens Medicine-Immunology and Allergy

CiteScore

6.40

自引率

8.10%

发文量

1285

审稿时长

17.75 days

期刊介绍： Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.