Does Every Strain of Pseudomonas aeruginosa Attack the Same? Results of a Study of the Prevalence of Virulence Factors of Strains Obtained from Different Animal Species in Northeastern Poland.

IF 3.3 3区医学 Q2 MICROBIOLOGY

Pathogens Pub Date : 2024-11-08 DOI:10.3390/pathogens13110979

Paweł Foksiński, Alicja Blank, Edyta Kaczorek-Łukowska, Joanna Małaczewska, Małgorzata Wróbel, Ewelina A Wójcik, Patrycja Sowińska, Nina Pietrzyk, Rafał Matusiak, Roman Wójcik

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引用次数: 0

Abstract

Background: Pseudomonas aeruginosa is a pathogen that causes infections in animals and humans, with veterinary implications including ear infections in dogs, respiratory diseases in cats, and mastitis in ruminants. In humans, it causes severe hospital-acquired infections, particularly in immunosuppressed patients. This study aimed to identify and assess the prevalence of specific virulence factors in Pseudomonas aeruginosa isolates.

Methods: We analyzed 98 Pseudomonas aeruginosa isolates from various animal samples (dogs, cats, ruminants, fowl) from northeastern Poland in 2019-2022 for virulence-related genes (toxA, exoU, exoT, exoS, lasB, plcN, plcH, pldA, aprA, gacA, algD, pelA, endA, and oprF) by PCR and assessed biofilm formation at 48 and 72 h. Genomic diversity was assessed by ERIC-PCR.

Results: The obtained results showed that all strains harbored the pelA gene (100%), while the lowest prevalence was found for pldA (24%) and exoU (36%). Regardless of the animal species, strong biofilm forming ability was prevalent among the strains after both 48 h (75%) and 72 h (74%). We obtained as many as 87 different genotyping profiles, where the dominant one was profile ERIC-48, observed in four strains.

Conclusions: No correlation was found between presence or absence of determined genes and the nature of infection. Similarly, no correlation was found between biofilm-forming genes and biofilm strength. The high genetic diversity indicates challenges for effective prevention, emphasizing the need for ongoing monitoring and research.

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铜绿假单胞菌的每种菌株都具有相同的攻击性吗？从波兰东北部不同动物物种中获取的菌株病毒性因子流行率的研究结果。

背景：铜绿假单胞菌是一种导致动物和人类感染的病原体，对兽医的影响包括狗的耳部感染、猫的呼吸道疾病和反刍动物的乳腺炎。在人类中，它会导致严重的医院感染，尤其是免疫抑制患者。本研究旨在确定和评估铜绿假单胞菌分离物中特定毒力因子的流行情况：我们通过 PCR 分析了 2019-2022 年从波兰东北部各种动物样本（狗、猫、反刍动物、禽类）中分离出的 98 株铜绿假单胞菌，检测了毒力相关基因（toxA、exoU、exoT、exoS、lasB、plcN、plcH、ppldA、aprA、gacA、algD、pelA、endA 和 oprF），并评估了 48 和 72 h 的生物膜形成情况：结果表明，所有菌株都携带 pelA 基因（100%），而 pldA（24%）和 exoU（36%）的流行率最低。无论动物种类如何，菌株在 48 小时（75%）和 72 小时（74%）后都普遍具有很强的生物膜形成能力。我们获得了多达 87 种不同的基因分型图谱，其中最主要的是 ERIC-48 图谱，在 4 株菌株中观察到：结论：在确定基因的存在与否和感染性质之间没有发现相关性。同样，生物膜形成基因与生物膜强度之间也没有相关性。基因的高度多样性表明有效预防面临挑战，强调了持续监测和研究的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathogens Medicine-Immunology and Allergy

CiteScore

6.40

自引率

8.10%

发文量

1285

审稿时长

17.75 days

期刊介绍： Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.