The Rhizobacterium Bacillus amyloliquefaciens MHR24 Has Biocontrol Ability Against Fungal Phytopathogens and Promotes Growth in Arabidopsis thaliana.

IF 4.1 2区生物学 Q2 MICROBIOLOGY

Microorganisms Pub Date : 2024-11-20 DOI:10.3390/microorganisms12112380

Mónica Hernández-Rodríguez, Diana Jasso-de Rodríguez, Francisco Daniel Hernández-Castillo, Ivana Moggio, Eduardo Arias, José Humberto Valenzuela-Soto, Alberto Flores-Olivas

{"title":"The Rhizobacterium Bacillus amyloliquefaciens MHR24 Has Biocontrol Ability Against Fungal Phytopathogens and Promotes Growth in Arabidopsis thaliana.","authors":"Mónica Hernández-Rodríguez, Diana Jasso-de Rodríguez, Francisco Daniel Hernández-Castillo, Ivana Moggio, Eduardo Arias, José Humberto Valenzuela-Soto, Alberto Flores-Olivas","doi":"10.3390/microorganisms12112380","DOIUrl":null,"url":null,"abstract":"A novel rhizobacteria Bacillus was isolated from rhizosphere of soil associated with tomato (Solanum lycopersicum L.) under open field conditions. The Bacillus amyloliquefaciens strain MHR24 (MHR24) is a promising biocontrol agent against several fungal phytopathogens. In this research, MHR24 was characterized by an effective antagonistic ability against Alternaria alternata (Aa), Botrytis cinerea (Bc), Fusarium oxysporum F1 (F1), F. oxysporum F2 (F2), F. oxysporum R3 (F3), and Sclerotinia sclerotiorum (Sc). In particular, MHR24 showed a strong inhibition via airborne volatiles against Bc, F3, Aa, and F2 fungal strains. MHR24 also showed elevated saline stress tolerance at 1% and 25% to NaCl and KCl. The molecular sequence analysis of 16S rDNA confirmed the identity of the isolate as Bacillus amyloliquefaciens strain MHR24. Bioassays on Arabidopsis thaliana Col-0 inoculated with MHR24 showed in in vitro conditions that MHR24 significantly increases the foliar and root area, while in growth chamber conditions, it strongly increases the dry shoot biomass of A. thaliana. The observed results indicate that B. amyloliquefaciens MHR24 has a broad-spectrum biocontrol against fungal phytopathogens and can be used as a biofertilizer and biocontrol agent to improve horticultural crops.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 11","pages":""},"PeriodicalIF":4.1000,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microorganisms","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/microorganisms12112380","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

A novel rhizobacteria Bacillus was isolated from rhizosphere of soil associated with tomato (Solanum lycopersicum L.) under open field conditions. The Bacillus amyloliquefaciens strain MHR24 (MHR24) is a promising biocontrol agent against several fungal phytopathogens. In this research, MHR24 was characterized by an effective antagonistic ability against Alternaria alternata (Aa), Botrytis cinerea (Bc), Fusarium oxysporum F1 (F1), F. oxysporum F2 (F2), F. oxysporum R3 (F3), and Sclerotinia sclerotiorum (Sc). In particular, MHR24 showed a strong inhibition via airborne volatiles against Bc, F3, Aa, and F2 fungal strains. MHR24 also showed elevated saline stress tolerance at 1% and 25% to NaCl and KCl. The molecular sequence analysis of 16S rDNA confirmed the identity of the isolate as Bacillus amyloliquefaciens strain MHR24. Bioassays on Arabidopsis thaliana Col-0 inoculated with MHR24 showed in in vitro conditions that MHR24 significantly increases the foliar and root area, while in growth chamber conditions, it strongly increases the dry shoot biomass of A. thaliana. The observed results indicate that B. amyloliquefaciens MHR24 has a broad-spectrum biocontrol against fungal phytopathogens and can be used as a biofertilizer and biocontrol agent to improve horticultural crops.

查看原文本刊更多论文

淀粉芽孢杆菌 MHR24 对真菌性植物病原体具有生物控制能力，并能促进拟南芥的生长。

在露地条件下，从与番茄（Solanum lycopersicum L.）相关的土壤根瘤中分离出一种新型根瘤菌芽孢杆菌。淀粉芽孢杆菌菌株 MHR24（MHR24）是一种很有前景的生物控制剂，可对抗多种真菌植物病原体。在这项研究中，MHR24 对交替交替孢霉（Aa）、灰霉病菌（Bc）、Fusarium oxysporum F1（F1）、F. oxysporum F2（F2）、F. oxysporum R3（F3）和 Sclerotinia sclerotiorum（Sc）具有有效的拮抗能力。特别是，MHR24 通过空气挥发物对 Bc、F3、Aa 和 F2 真菌菌株有很强的抑制作用。MHR24 对 NaCl 和 KCl 的耐盐性也有所提高，分别为 1%和 25%。16S rDNA 的分子序列分析证实了该分离物为淀粉芽孢杆菌 MHR24 菌株。对接种了 MHR24 的拟南芥 Col-0 进行的生物测定表明，在离体条件下，MHR24 能显著增加拟南芥的叶面积和根面积，而在生长室条件下，它能显著增加拟南芥的干芽生物量。观察结果表明，B. amyloliquefaciens MHR24 对真菌植物病原体具有广谱的生物防治作用，可用作生物肥料和生物防治剂来改良园艺作物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Microorganisms Medicine-Microbiology (medical)

CiteScore

7.40

自引率

6.70%

发文量

2168

审稿时长

20.03 days

期刊介绍： Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.