Metabolic Effects of Loquat Juice (Eriobotrya japonica Lindl Mkarkeb Variety) on Lipid Homeostasis, Liver Steatosis, and Oxidative Stress in Hyperlipidemic Mice Fed a High-Fat-High-Fructose Diet.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2024-11-02 DOI:10.3390/metabo14110592

Imane Mokhtari, Thamer Aljutaily, Huda Aljumayi, Khadija S Radhi, Abdulkarim S Almutairi, Hassan Barakat, Ibrahim Khalifa, Souliman Amrani, Hicham Harnafi

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引用次数: 0

Abstract

Background: Loquat fruit is consumed for its flavorful taste and a rich array of health-promoting compounds like phenolics, flavonoids, and carotenoids. This study aimed at the biochemical characterization of fresh juice from the Moroccan Mkarkeb variety of loquat and evaluating its effects on lipid homeostasis and liver steatosis in hyperlipidemic mice.

Methods: The biochemical characterization followed AOAC methods. In vivo study involved hyperlipidemic mice fed a high-fat, high-fructose diet for 6 weeks and treated with loquat juice at 3.5 and 7 mL kg^-1 or fenofibrate at 4 mg·kg^-1. The concentrations of lipids in plasma, liver, adipose tissue, feces, and bile and blood glucose levels were quantified. Liver steatosis was visually examined and confirmed histologically, and liver injury markers (AST, ALT, ALP, LDH, and TB) were measured. Liver oxidative stress was assessed by measuring MDA content and antioxidative enzyme activities.

Results: Our findings indicate that fresh loquat juice is poor in fat and protein and contains moderate sugars with a low energy value (40.82 ± 0.25 kcal/100 g). It is also rich in minerals, vitamin C, phenolic acids, flavonoids, and carotenoids. The juice effectively restored lipid metabolism by enhancing reverse cholesterol transport and lowering LDL-cholesterol, triglycerides, and the atherogenic index. The studied juice decreases blood glucose and prevents weight gain and lipid accumulation in the liver and adipose tissue. The juice prevents lipotoxicity-induced liver injury, corrects toxicity markers, and improves the liver's morphological and histological structures. It also reduces oxidative stress by lowering MDA and activating SOD and catalase.

Conclusions: The juice holds high nutritional and medicinal value, potentially preventing lipid disorders and cardiovascular issues.

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枇杷汁（Eriobotrya japonica Lindl Mkarkeb Variety）对高脂高果糖饮食高血脂小鼠血脂平衡、肝脏脂肪变性和氧化应激的代谢影响

背景：枇杷果味道鲜美，含有丰富的酚类、类黄酮和类胡萝卜素等促进健康的化合物，因此深受人们喜爱。本研究旨在对来自摩洛哥 Mkarkeb 品种枇杷的新鲜果汁进行生化鉴定，并评估其对高脂血症小鼠血脂稳态和肝脏脂肪变性的影响：方法：生化表征按照 AOAC 方法进行。高脂血症小鼠以高脂肪、高果糖饮食喂养 6 周，并接受 3.5 和 7 mL kg-1 的枇杷汁或 4 mg-kg-1 的非诺贝特治疗。对血浆、肝脏、脂肪组织、粪便和胆汁中的脂质浓度以及血糖水平进行了量化。对肝脏脂肪变性进行肉眼检查和组织学确认，并测量肝损伤指标（AST、ALT、ALP、LDH 和 TB）。肝脏氧化应激通过测量 MDA 含量和抗氧化酶活性进行评估：结果：我们的研究结果表明，新鲜枇杷汁的脂肪和蛋白质含量较低，糖分含量适中，能量值较低（40.82 ± 0.25 千卡/100 克）。它还含有丰富的矿物质、维生素 C、酚酸、类黄酮和类胡萝卜素。果汁能增强胆固醇的逆向运输，降低低密度脂蛋白胆固醇、甘油三酯和动脉粥样硬化指数，从而有效恢复脂质代谢。所研究的果汁能降低血糖，防止体重增加以及肝脏和脂肪组织中的脂质积累。果汁能防止脂肪毒性引起的肝损伤，纠正毒性标志物，改善肝脏的形态和组织结构。它还能降低 MDA，激活 SOD 和过氧化氢酶，从而减少氧化应激：果汁具有很高的营养和药用价值，可预防血脂紊乱和心血管问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.