The Effects of Agaro-Oligosaccharides Produced by Marine Bacteria (Rheinheimera sp. (HY)) Possessing Agarose-Degrading Enzymes on Myotube Function.

IF 4.9 2区医学 Q1 CHEMISTRY, MEDICINAL

Marine Drugs Pub Date : 2024-11-14 DOI:10.3390/md22110515

Youshi Huang, Takuya Hirose, Jyh-Ming Tsai, Katsuya Hirasaka

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引用次数: 0

Abstract

Agarase and its metabolites are reported to have applications in a variety of fields, but there have been few studies of the effects of agaro-oligosaccharide hydrolysate on muscle function. In this study, we analyzed the functionality of agarase and its metabolites in bacteria isolated from seawater. A bacterium with agar-degrading activity was isolated from Shimabara, Nagasaki, Japan. Through 16S rRNA sequence alignment, it was identified as being closely related to Rheinheimera sp. WMF-1 and was provisionally named Rheinheimera sp. (HY). Crude enzymes derived from this bacterium demonstrated an ability to hydrolyze various polysaccharides, including agar, agarose, and starch, with the highest specificity observed for agarose. The optimum pH and temperature were pH 10 and 50 °C. A glycoside bond specificity analysis of enzymatic activity indicated the cleavage of the α-linkage. Next, we investigated the functional effects of agaro-oligosaccharides on C2C12 myotubes. Treatment with 10-30 kDa oligosaccharides significantly increased the hypertrophy rate, diameter, and expression of myosin heavy-chain genes in C2C12 myotubes. These results indicate that the agaro-oligosaccharides produced by the enzymes identified in this study improve muscle mass, suggesting their potential contribution to muscle function.

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海洋细菌（Rheinheimera sp.具有琼脂糖降解酶的海洋细菌（Rheinheimera sp.

据报道，琼脂糖酶及其代谢物可应用于多个领域，但有关琼脂寡糖水解物对肌肉功能影响的研究却很少。在这项研究中，我们分析了从海水中分离出来的细菌中的琼脂糖酶及其代谢物的功能。我们从日本长崎岛原分离到了一种具有琼脂降解活性的细菌。通过 16S rRNA 序列比对，确定它与 Rheinheimera sp.从该细菌中提取的粗酶证明能够水解多种多糖，包括琼脂、琼脂糖和淀粉，其中对琼脂糖的特异性最高。最佳 pH 值和温度分别为 pH 值 10 和 50 °C。对酶活性进行的糖苷键特异性分析表明，α-连接可被裂解。接下来，我们研究了琼脂寡糖对 C2C12 肌细胞的功能影响。用10-30 kDa低聚糖处理C2C12肌细胞，可明显增加其肥大率、直径和肌球蛋白重链基因的表达。这些结果表明，本研究发现的酶产生的琼脂寡糖能提高肌肉质量，表明它们对肌肉功能有潜在贡献。

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来源期刊

Marine Drugs 医学-医药化学

CiteScore

9.60

自引率

14.80%

发文量

671

审稿时长

1 months

期刊介绍： Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.