{"title":"Genome-Based Mining of Carpatamides I-M and Their Candidate Biosynthetic Gene Cluster.","authors":"Shu-Mei Shen, Yun-Chang Xie, Li-Rong Tu, Miao-Er Wu, Yan-Min Wang, Chun-Hui Song, Yu-Hui Sun, Ming-He Luo","doi":"10.3390/md22110521","DOIUrl":null,"url":null,"abstract":"<p><p>Chemically investigating the marine-derived <i>Streptomyces parvus</i> 1268 led to the isolation of a new compound of carpatamide I (<b>1</b>). Subsequent genomic analysis identified its candidate biosynthetic gene cluster <i>ctd</i> of approximately 44 kb. In order to obtain more carpatamide derivatives, we conducted the upregulation of Ctd14, which is a positive regulator, and obtained improvement of carpatamide I and four new compounds of carpatamides J-M (<b>2</b>-<b>5</b>). The structures of the aforementioned five new isolates were identified by a combination of ESI-HRMS as well as one-dimensional (1D) and two-dimensional (2D) spectral NMR datasets. Bioassay results showed that compounds <b>1</b>-<b>5</b> displayed anti-inflammatory activity and weak cytotoxicity against cell lines of A549, HT-29, and HepG2.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9000,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595529/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine Drugs","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/md22110521","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
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Abstract
Chemically investigating the marine-derived Streptomyces parvus 1268 led to the isolation of a new compound of carpatamide I (1). Subsequent genomic analysis identified its candidate biosynthetic gene cluster ctd of approximately 44 kb. In order to obtain more carpatamide derivatives, we conducted the upregulation of Ctd14, which is a positive regulator, and obtained improvement of carpatamide I and four new compounds of carpatamides J-M (2-5). The structures of the aforementioned five new isolates were identified by a combination of ESI-HRMS as well as one-dimensional (1D) and two-dimensional (2D) spectral NMR datasets. Bioassay results showed that compounds 1-5 displayed anti-inflammatory activity and weak cytotoxicity against cell lines of A549, HT-29, and HepG2.
期刊介绍：
Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.
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