Anna Portugalov, Gaia Peled, Sharon Zorin, Irit Akirav
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引用次数: 0
Abstract
Post-traumatic stress disorder (PTSD) is a debilitating neuropsychiatric condition closely linked to neuroinflammation, with a higher prevalence in women. Cannabidiol (CBD), a non-psychoactive cannabinoid, has shown promise as a potential treatment for PTSD. In this study, we used a PTSD model in which female rats were subjected to a severe foot shock followed by contextual situational reminders (SRs). Testing was conducted one month after exposure. The rats received daily CBD injections for three weeks during the SRs, from days 7 to 28. Two days after the final SR, the rats underwent five extinction trials, followed by the forced swim test (FST). After a five-day rest period, the rats were sacrificed, and brain tissues from the medial prefrontal cortex (mPFC) and ventral subiculum (vSUB) were analyzed for inflammatory markers. Chronic CBD treatment reversed impairments in fear extinction caused by shock and SR. It also reduced learned helplessness in the FST and decreased the upregulation of mPFC-il1β induced by shock and SRs. Additionally, exposure to shock and SRs downregulated mPFC-il6 while upregulating vSUB-il6. CBD treatment further downregulated il6 expression in the vSUB compared to the vehicle groups. Our findings show that CBD effectively inhibited the development of PTSD-like behaviors and suppressed neuroinflammation in the mPFC.
创伤后应激障碍(PTSD)是一种令人衰弱的神经精神疾病,与神经炎症密切相关,女性发病率较高。大麻二酚(CBD)是一种非精神活性大麻素,已显示出治疗创伤后应激障碍的潜力。在这项研究中,我们使用了一种创伤后应激障碍模型,让雌性大鼠受到严重的脚部冲击,然后进行情境提醒(SR)。暴露一个月后进行测试。在SR期间的第7天至第28天,大鼠每天接受CBD注射,为期三周。在最后一次情境提醒两天后,大鼠接受了五次消退试验,随后进行了强迫游泳试验(FST)。休息五天后,大鼠被处死,并对内侧前额叶皮层(mPFC)和腹侧子网(vSUB)的脑组织进行炎症标记物分析。慢性 CBD 治疗逆转了休克和 SR 引起的恐惧消退障碍。它还减少了FST中的习得性无助,并降低了冲击和SR诱导的mPFC-il1β上调。此外,受到冲击和SRs会下调mPFC-il6,同时上调vSUB-il6。与车辆组相比,CBD处理进一步下调了vSUB中il6的表达。我们的研究结果表明,CBD能有效抑制创伤后应激障碍样行为的发展,并抑制mPFC的神经炎症。
BiomoleculesBiochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
9.40
自引率
3.60%
发文量
1640
审稿时长
18.28 days
期刊介绍:
Biomolecules (ISSN 2218-273X) is an international, peer-reviewed open access journal focusing on biogenic substances and their biological functions, structures, interactions with other molecules, and their microenvironment as well as biological systems. Biomolecules publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.