Global phylogeography and genomic characterization of Vibrio parahaemolyticus infections in Jilin province, China (2016–2022)

Abstract

Vibrio parahaemolyticus is a critical foodborne pathogen causing gastroenteritis worldwide. The occurrence of transmission and outbreaks attributed to V. parahaemolyticus has exhibited a notable upward trend during the past two decades. However, comprehensive information on this pathogen in inland cities in China remains scarce. This study shed light on the molecular characteristics, genetic associations, and significant transmission risks through foodborne and fecal-oral routes of 115 V. parahaemolyticus strains obtained from nine inland cities in Jilin Province. Strains were divided into 90 sequence types (STs), with 41 STs that were novel. The predominant sequence type was ST3 (14.78 %, 17/115). The strains exhibited the highest resistance rates to cefazolin and ampicillin. A total of ninety-four antibiotic resistance genes (ARGs) categorized groups based on antibiotic class were identified. The tet(34) (112/115, 97.39 %) and bla_CARB (114/115, 99.13 %) genes responsible for tetracycline and β-lactams resistance were present in most isolates. Interestingly, V. parahaemolyticus with the tet(34) gene may not be resistant to tetracycline. Strains with strong biofilm formation ability carry more resistance genes. Atypical virulence genes and virulence genome islands (VPaI) were also identified in the isolated strains. All strains encoded type III secretion system 1 (T3SS1), while 114 isolates encoded type VI secretion system 2 (T6SS2). Notably, T6SS1 was present in 59.55 % of food strains, and pathogenicity genomic islands VPaI-1 to VPaI-5 were found in food-associated isolates. Based on the results of the phylogenetic analysis, clinical strains were closely related within a single cluster, which refers to strains that are more similar to each other than to those outside the group based on specific genetic profiles, while the food isolates were highly diverse. Furthermore, some isolates included in our study indicated potential transmission possibly through sharing of some SNPs between food and clinical-positive V. parahaemolyticus strains from different countries. The study elucidates the genetic characteristics, diversity, and virulence potential of V. parahaemolyticus isolates, thereby enhancing the understanding of the potential risks associated with the cross-border transmission of this pathogen.