A novel aptamer-based photoelectrochemical sensor for zearalenone detection: Integration of g-C3N4/BiOBr with in situ growth Ag2S quantum dots

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2024-11-23 DOI:10.1016/j.bioelechem.2024.108853

Pengfei Yu , Jiaqi Cui , Jun Yang , Mati Ullah Khan , Lina Yang , Shanshan Li , Meixin Li , Shuang Liu , Ling Kong , Zhiwei Chen

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引用次数: 0

Abstract

Zearalenone (ZEN), a secondary metabolite mycotoxin primarily synthesized by Fusarium species and prevalent in cereal grains, exerts estrogenic effects that could induce reproductive toxicity and teratogenic outcomes. To enhance the precision of ZEN detection, we have developed an innovative photoelectrochemical (PEC) aptamer-based sensor employing in situ growth silver sulfide (Ag₂S) quantum dots-sensitized graphitic carbon nitride/bismuth oxybromide (g-C₃N₄/BiOBr) heterojunction. The g-C₃N₄/BiOBr composite exhibits robust structural stability and straightforward synthesis. In situ growth Ag₂S quantum dots could significantly amplify the signal, thereby elevating the sensor’s sensitivity. The sensor utilizes ZEN-specific aptamers for target-specific binding, ensuring high selectivity. Within the concentration range of 0.001 to 100 ng·mL⁻¹, the photocurrent response demonstrated a significant linear correlation with the logarithmic scale of ZEN concentration, with a detection limit reaching 0.122 pg·mL⁻¹. This PEC aptamer sensor exhibits excellent stability, reproducibility, and selectivity, which is expected to be used as an effective analytical tool for ZEN detection in practical applications.

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一种用于检测玉米赤霉烯酮的基于适配体的新型光电化学传感器：将 g-C3N4/BiOBr 与原位生长的 Ag2S 量子点相结合

玉米赤霉烯酮（Zearalenone，ZEN）是一种次生代谢物霉菌毒素，主要由镰刀菌类合成，普遍存在于谷物中，具有雌激素效应，可诱发生殖毒性和致畸结果。为了提高 ZEN 的检测精度，我们开发了一种创新的基于光电化学（PEC）的传感器，采用原位生长硫化银（Ag2S）量子点敏化氮化石墨碳/氧溴化铋（g-C3N4/BiOBr）异质结。g-C3N4/BiOBr 复合材料结构稳定，合成简单。原位生长的 Ag2S 量子点可以显著放大信号，从而提高传感器的灵敏度。该传感器利用 ZEN 特异性适配体进行目标特异性结合，确保了高选择性。在 0.001 至 100 ng-mL-1 的浓度范围内，光电流响应与 ZEN 浓度的对数刻度呈显著的线性相关，检测限达到 0.122 pg-mL-1。这种 PEC 合酶传感器具有极佳的稳定性、重现性和选择性，有望在实际应用中成为检测 ZEN 的有效分析工具。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.