Immunoprotective efficacy of Escherichia coli-derived outer membrane vesicles displaying PlpE protein of Pasteurella multocida

IF 4.5 3区医学 Q2 IMMUNOLOGY

Vaccine Pub Date : 2024-11-26 DOI:10.1016/j.vaccine.2024.126532

Yajuan Li , Liyi Chen , Junfang Xiao , Keyu Feng , Xinheng Zhang , Yung-Fu Chang , Qingmei Xie

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引用次数: 0

Abstract

Pasteurella multocida (P. multocida), a pathogenic bacterium known to induce duck cholera, stands as a significant contributor to bacterial diseases afflicting the duck industry, causing substantial annual economic losses on a global scale. In this study, the genes encoding the lipoproteins PlpE of P. multocida strain PMWSG-4 was cloned, inserted into the pBAD-ClyA vector, and the recombinant outer membrane vesicles (OMVs) fused with PlpE antigen of P. multocida was expressed by Escherichia coli (E. coli). Ducks immunized with OMV-PlpE had significantly (P < 0.001) increased production of antigen-specific antibodies. Moreover, at 28 days post-immunization, the expression of genes associated with immune response, including interleukin (IL)-2, IL-4, IL-10, and interferon (IFN)-γ in the spleen tissue of immunized ducks were significantly (P < 0.001) up-regulated compared to unimmunized ducks in the control group. And the active serum had significant bactericidal effects against the PMWSG-4 strain (P < 0.001). The protective efficacy of the vaccines was evaluated by leg muscle challenge with 20 LD50 doses of P. multocida, with the recombinant OMV-PlpE conferring 100 % protection. Histopathological examination and tissue bacterial load detection revealed that OMV-PlpE mitigated tissue damage and bacterial colonization to a statistically significant extent (P < 0.001). These findings serve as a valuable reference for the development of vaccines against P. multocida.

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显示多杀性巴氏杆菌 PlpE 蛋白的大肠杆菌衍生外膜囊泡的免疫保护功效

多杀性巴氏杆菌（P. multocida）是一种已知可诱发鸭霍乱的致病菌，是困扰养鸭业的细菌性疾病的主要致病菌，每年在全球范围内造成巨大的经济损失。本研究克隆了多杀性巴氏杆菌菌株 PMWSG-4 的脂蛋白 PlpE 编码基因，将其插入 pBAD-ClyA 载体，并用大肠杆菌（E. coli）表达了与多杀性巴氏杆菌 PlpE 抗原融合的重组外膜囊泡（OMVs）。用 OMV-PlpE 免疫的鸭子产生的抗原特异性抗体显著增加（P < 0.001）。此外，免疫后 28 天，免疫鸭脾组织中与免疫应答相关的基因，包括白细胞介素（IL）-2、IL-4、IL-10 和干扰素（IFN）-γ 的表达，与未免疫的对照组鸭相比，有明显的上调（P < 0.001）。活性血清对 PMWSG-4 株有明显的杀菌作用（P < 0.001）。用20 LD50剂量的多杀性猪嗜血杆菌挑战腿部肌肉，评估疫苗的保护效力，重组OMV-PlpE可提供100%的保护。组织病理学检查和组织细菌负荷检测显示，OMV-PlpE 在统计学上显著减轻了组织损伤和细菌定植（P < 0.001）。这些研究结果为开发多杀菌素疫苗提供了宝贵的参考。

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来源期刊

Vaccine 医学-免疫学

CiteScore

8.70

自引率

5.50%

发文量

992

审稿时长

131 days

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