Chemical activation of ABA signaling in grapevine through the iSB09 and AMF4 ABA receptor agonists enhances water use efficiency.

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Mar Bono, Raul Ferrer-Gallego, Alicia Pou, Maria Rivera-Moreno, Juan L Benavente, Cristian Mayordomo, Leonor Deis, Pablo Carbonell-Bejerano, Gaston A Pizzio, David Navarro-Payá, José Tomás Matus, Jose Miguel Martinez-Zapater, Armando Albert, Diego S Intrigliolo, Pedro L Rodriguez
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Abstract

Grapevine (Vitis vinifera L.) is the world's third most valuable horticultural crop, and the current environmental scenario is massively shifting the grape cultivation landscape. The increase in heatwaves and drought episodes alter fruit ripening, compromise grape yield and vine survival, intensifying the pressure on using limited water resources. ABA is a key phytohormone that reduces canopy transpiration and helps plants to cope with water deficit. However, the exogenous application of ABA is impractical because it suffers fast catabolism, and UV-induced isomerization abolishes its bioactivity. Consequently, there is an emerging field for developing molecules that act as ABA receptor agonists and modulate ABA signaling but have a longer half-life. We have explored the foliar application of the iSB09 and AMF4 agonists in the two grapevine cultivars cv. 'Bobal' and 'Tempranillo' to induce an ABA-like response to facilitate plant adaptation to drought. The results indicate that iSB09 and AMF4 act through the VviPYL1-like, VviPYL4-like, and VviPYL8-like ABA receptors to trigger stomatal closure, reduce plant transpiration, and increase water use efficiency. Structural and bioinformatic analysis of VviPYL1 in complex with ABA or these agonists revealed key structural determinants for efficient ligand binding, providing a mechanistic framework to understand receptor activation by the ligands. Physiological analyses further demonstrated that iSB09 has a more sustained effect on reducing transpiration than ABA, and agonist spraying of grapevine leaves protected PSII during drought stress. These findings offer innovative approaches to strengthen the vine's response to water stress and reduce plant consumptive water use under limited soil water conditions.

通过 iSB09 和 AMF4 ABA 受体激动剂化学激活葡萄中的 ABA 信号,提高水分利用效率。
葡萄(Vitis vinifera L.)是世界第三大最有价值的园艺作物,而当前的环境状况正在极大地改变葡萄种植的格局。热浪和干旱的增加改变了果实的成熟期,影响了葡萄的产量和葡萄藤的存活率,加剧了利用有限水资源的压力。ABA 是一种关键的植物激素,能降低树冠蒸腾作用,帮助植物应对缺水问题。然而,外源施用 ABA 并不现实,因为 ABA 会被快速分解,紫外线诱导的异构化也会削弱其生物活性。因此,开发可作为 ABA 受体激动剂、调节 ABA 信号、但半衰期较长的分子成为一个新兴领域。我们对 iSB09 和 AMF4 激动剂在两个葡萄栽培品种 "Bobal "和 "Tempranillo "中的叶面应用进行了探索,以诱导类似 ABA 的反应,促进植物对干旱的适应。结果表明,iSB09和AMF4通过VviPYL1-like、VviPYL4-like和VviPYL8-like ABA受体发挥作用,引发气孔关闭,降低植物蒸腾作用,提高水分利用效率。对 VviPYL1 与 ABA 或这些激动剂复合物的结构和生物信息学分析揭示了有效结合配体的关键结构决定因素,为理解配体激活受体提供了一个机理框架。生理学分析进一步证明,iSB09 比 ABA 更能持续降低蒸腾作用,在干旱胁迫期间,喷洒激动剂能保护葡萄叶片的 PSII。这些发现为加强葡萄树对水分胁迫的反应和减少植物在有限土壤水分条件下的耗水量提供了创新方法。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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