High throughput cell mechanotyping of cell response to cytoskeletal modulations using a microfluidic cell deformation system

IF 2.3 4区工程技术 Q2 INSTRUMENTS & INSTRUMENTATION

Microfluidics and Nanofluidics Pub Date : 2024-11-26 DOI:10.1007/s10404-024-02774-4

Ian M. Smith, Jeanine A. Ursitti, Sai Pranav Majeti Venkata, Nikka Givpoor, Megan B. Stemberger, Autumn Hengen, Shohini Banerjee, Khaled Hached, Siem van der Laan, Joseph Stains, Stuart S. Martin, Christopher Ward, Kimberly M. Stroka

引用次数: 0

Abstract

Cellular mechanical properties influence cellular functions across pathological and physiological systems. The observation of these mechanical properties is limited in part by methods with a low throughput of acquisition or with low accessibility. To overcome these limitations, we have designed, developed, validated, and optimized a microfluidic cellular deformation system (MCDS) capable of mechanotyping suspended cells on a population level at a high throughput rate of ~ 300 cells per second. The MCDS provides researchers with a viable method for efficiently quantifying cellular mechanical properties towards defining prognostic implications of mechanical changes in pathology or screening drugs to modulate cytoskeletal integrity.

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利用微流体细胞变形系统对细胞对细胞骨架调节的反应进行高通量细胞机械分型

细胞机械特性影响着病理和生理系统中的细胞功能。对这些机械特性的观察部分受限于采集通量低或可及性低的方法。为了克服这些限制，我们设计、开发、验证并优化了一种微流体细胞变形系统（MCDS），该系统能够以每秒约 300 个细胞的高通量速率在群体水平上对悬浮细胞进行机械分型。MCDS 为研究人员提供了一种有效量化细胞机械特性的可行方法，可用于确定病理中机械变化的预后影响或筛选调节细胞骨架完整性的药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microfluidics and Nanofluidics 工程技术-纳米科技

CiteScore

4.80

自引率

3.60%

发文量

审稿时长

2 months

期刊介绍： Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).