Evaluation of CTX-I, CTX-II, TIMP-I, MMP-9 and PIICP in the population of working German Shepherd dogs in Slovakia.

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES

Veterinarni Medicina Pub Date : 2024-10-30 eCollection Date: 2024-10-01 DOI:10.17221/127/2023-VETMED

Scarlett Maresova, Tomas Liptak, Aladar Madari, Patrik Zeleznik, Csilla Tothova, Maria Kuricova

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Abstract

The aim of this study was to determine the reference values of cartilage damage biomarkers in the blood and urine in 76 clinically healthy German Shepherd dogs. We grouped the dogs into 4 groups by age. All the groups were consistently made up of 19 dogs. The mean age and average body weight were established for all the dog groups. The blood was collected from the vena cephalica antebrachii or the vena saphena medialis and the urine sample was taken by us-guided cystocenthesis. The biomarkers were determined using quantitative sandwich ELISA kits. The mean values of the biomarkers ± SD were, for the urine biomarkers, CTX-I 3.29 ± 1.16 ng/ml, CTX-II 1 993.95 ± 777.04 ng/ml, and TIMP-I 392.80 ± 160.56 ng/ml, and for the blood biomarkers, MMP-9 89.85 ± 50.21 ng/ml and PIICP 19.19 ± 7.33 ng/ml. Based on the obtained values of the mean 95%, we expect a standard for CTX-I 5.05 ng/ml, CTX-II 3 204.26 ng/ml, TIMP-I 606.64 ng/ml, MMP-9 187.93 ng/ml, and PIICP 31.71 ng/ml.

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评估斯洛伐克工作德国牧羊犬群体中的 CTX-I、CTX-II、TIMP-I、MMP-9 和 PIICP。

本研究旨在确定 76 只临床健康德国牧羊犬血液和尿液中软骨损伤生物标志物的参考值。我们将这些狗按年龄分为 4 组。所有组别均由 19 只狗组成。我们确定了各组狗的平均年龄和平均体重。我们从头静脉前部或静脉内侧采集血液，并通过我们引导的膀胱穿刺术采集尿液样本。生物标志物采用夹心酶联免疫吸附法定量检测。尿液生物标记物的平均值（± SD）为 CTX-I 3.29 ± 1.16 ng/ml、CTX-II 1 993.95 ± 777.04 ng/ml、TIMP-I 392.80 ± 160.56 ng/ml；血液生物标记物的平均值（± SD）为 MMP-9 89.85 ± 50.21 ng/ml、PIICP 19.19 ± 7.33 ng/ml。根据所获得的平均值 95%，我们预计 CTX-I 为 5.05 ng/ml，CTX-II 为 3 204.26 ng/ml，TIMP-I 为 606.64 ng/ml，MMP-9 为 187.93 ng/ml，PIICP 为 31.71 ng/ml。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinarni Medicina Veterinary Sciences-兽医学

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

18-36 weeks

期刊介绍： The journal Veterinarni Medicina publishes in English original papers, short communications, critical reviews and case reports from all fields of veterinary and biomedical sciences.