In Vitro Efficacy of Photodynamic Antimicrobial Chemotherapy with TONS504 Using Blue Light Against Pseudomonas aeruginosa.

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Yonago acta medica Pub Date : 2024-10-07 eCollection Date: 2024-11-01 DOI:10.33160/yam.2024.11.001

Tomoya Furujo, Norihiko Ito, Kazuki Harada, Isao Sakata, Tomohiro Osaki

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引用次数: 0

Abstract

Background: Pseudomonas aeruginosa is an important causative agent of bacterial keratitis. This study investigates the antibacterial effect of photodynamic antimicrobial chemotherapy (PACT) with the chlorin derivative TONS504 (TONS504-PACT) and a blue light-emitting diode (LED) on P. aeruginosa in vitro. It also explores the synergistic effects of combining TONS504-PACT with EDTA, and compares the antibacterial effects of TONS504-PACT using blue light with those of previously reported red light.

Methods: To evaluate the antibacterial effects of TONS504-PACT using blue light on P. aeruginosa, PACT with TONS504 (0-100 mg/L) was applied using a 405 nm LED at 0-30 J/cm² to P. aeruginosa (10^7-8 CFU/mL). The antibacterial effects were assessed by calculating the survival fraction. Subsequently, to compare the effects of TONS504-PACT using blue light with those of using red light, PACT with TONS504 (10 or 100 mg/L) was conducted using a 405 or 660 nm LED at 30 J/cm² and evaluated in the same manner. Finally, to investigate the synergistic antibacterial effects of TONS504-PACT with EDTA, PACT with TONS504 (0-100 mg/L) was applied using a 405 nm LED at 5 J/cm², both with and without EDTA (0.015 M), and its antibacterial effects were similarly assessed.

Results: TONS504-PACT using blue light decreased the survival of P. aeruginosa, depending on the light intensity. The greatest antibacterial effect was observed at 10 mg/L and 30 J/cm², showing a survival fraction of 3.5 × 10^-5. TONS504-PACT demonstrated a significantly greater antibacterial effect using blue light than that demonstrated using red light at 10 mg/L, though not at 100 mg/L. The combination of TONS504-PACT using blue light with EDTA significantly enhanced antibacterial effects at all concentrations.

Conclusion: These findings confirm the effectiveness of TONS504-PACT using blue light against P. aeruginosa and suggest its potential as a treatment for bacterial keratitis.

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使用蓝光的 TONS504 光动力抗菌化疗对铜绿假单胞菌的体外疗效

背景：铜绿假单胞菌是细菌性角膜炎的重要致病菌：铜绿假单胞菌是细菌性角膜炎的重要致病菌。本研究探讨了氯素衍生物 TONS504（TONS504-PACT）和蓝色发光二极管（LED）的光动力抗菌化疗（PACT）在体外对铜绿假单胞菌的抗菌效果。本研究还探讨了 TONS504-PACT 与 EDTA 结合的协同效应，并比较了 TONS504-PACT 使用蓝光与之前报道的红光的抗菌效果：为了评估 TONS504-PACT 使用蓝光对铜绿假单胞菌的抗菌效果，使用波长为 405 nm、功率为 0-30 J/cm2 的 LED 对铜绿假单胞菌（107-8 CFU/mL）照射含有 TONS504 的 PACT（0-100 mg/L）。通过计算存活率来评估抗菌效果。随后，为了比较使用蓝光的 TONS504-PACT 和使用红光的 TONS504-PACT 的效果，使用 30 J/cm2 的 405 或 660 nm LED 对 TONS504（10 或 100 mg/L）进行了 PACT，并以同样的方式进行了评估。最后，为了研究 TONS504-PACT 与 EDTA 的协同抗菌效果，使用 5 J/cm2 的 405 nm LED 对 TONS504（0-100 mg/L）与 EDTA（0.015 M）进行了 PACT，并同样评估了其抗菌效果：结果：使用蓝光的 TONS504-PACT 可降低铜绿假单胞菌的存活率，具体取决于光照强度。在 10 mg/L 和 30 J/cm2 的条件下，抗菌效果最佳，存活率为 3.5 × 10-5。在 10 毫克/升的条件下，TONS504-PACT 使用蓝光的抗菌效果明显高于使用红光的抗菌效果，但在 100 毫克/升的条件下则不然。将蓝光 TONS504-PACT 与乙二胺四乙酸结合使用，可显著增强所有浓度下的抗菌效果：这些研究结果证实了 TONS504-PACT 使用蓝光对铜绿假单胞菌的有效性，并表明其具有治疗细菌性角膜炎的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Yonago acta medica MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Yonago Acta Medica (YAM) is an electronic journal specializing in medical sciences, published by Tottori University Medical Press, 86 Nishi-cho, Yonago 683-8503, Japan. The subject areas cover the following: molecular/cell biology; biochemistry; basic medicine; clinical medicine; veterinary medicine; clinical nutrition and food sciences; medical engineering; nursing sciences; laboratory medicine; clinical psychology; medical education. Basically, contributors are limited to members of Tottori University and Tottori University Hospital. Researchers outside the above-mentioned university community may also submit papers on the recommendation of a professor, an associate professor, or a junior associate professor at this university community. Articles are classified into four categories: review articles, original articles, patient reports, and short communications.