Leptin/Melanocortin Pathway in Cholelithiasis Patients: A Diagnostic Perspective?

IF 1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Protein and Peptide Letters Pub Date : 2024-11-22 DOI:10.2174/0109298665343979241025114114

Tugba Agbektas, Gülşen Güçlü, Ayça Tas, Esma Ozmen, Ömer Topçu, Süleyman Aydin, Yavuz Sılıg

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引用次数: 0

Abstract

Background: Cholelithiasis is the most prevalent inflammatory condition of the gallbladder. The regulation of biological processes, including energy homeostasis, and control of body weight are key mechanisms that the leptin and melanocortin pathways play a role in. Cholelithiasis is the most prevalent inflammatory condition of the gallbladder. There are various risk factors for the development of gallstone disease, especially weight gain, and obesity is just one of them. This risk factor can be minimized by maintaining appetite and energy balance. Here, leptin and melanocortin pathways are the key mechanisms in maintaining appetite and energy homeostasis.

Objectives: The aim of this study was to investigate the relationship between the levels of LEP, LEPR, TrkB, BDNF, POMC, and MC4R proteins in patients with Cholelithiasis. This study aims to determine the relationship between LEP, LEPR, TrkB, BDNF, POMC, and MC4P protein levels, which play a role in maintaining appetite and energy homeostasis, and cholelithiasis.

Methods: This study examined 44 patients diagnosed with Cholelithiasis and 44 healthy control subjects who had not previously been diagnosed with any form of Cholelithiasis. The levels of leptin (LEP), Leptin Binds To Leptin Receptors (LEPR), Tropomyosin Receptor Kinase B (TrkB), Brain-Derived Neurotrophic Factor (BDNF), Pro-OpioMelanoCortin (POMC), and Melanocortin- 4 Receptors (MC4R) molecules were analyzed using the Enzyme-Linked Immunosorbent Assay (ELISA) method. The results were analyzed using the SPSS Software (Version 22.0) program and GraphPad Prism 8.0.1 software.

Results: The study found a statistically significant decrease (p < 0.05) in MC4R, TrkB, BDNF, and POMC protein levels in Cholelithiasis patients compared to the control group. There was no statistically significant difference in LEP and LEPR concentration values between the two groups (p=0.247, p=0.674).

Conclusion: The proteins MC4R, TrkB, BDNF, and POMC, which are involved in the leptin and melanocortin pathways may play a significant role in Cholelithiasis disease. However, more detailed research on the relevant proteins is needed. Nevertheless, this research will guide new studies.

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胆石症患者的瘦素/黑皮素通路：诊断视角？

背景：胆石症是最常见的胆囊炎性疾病。瘦素和黑色素皮质素通路在调节能量平衡等生物过程和控制体重的关键机制中发挥作用。胆石症是胆囊最常见的炎症。胆石症的发病有多种风险因素，尤其是体重增加，而肥胖只是其中之一。保持食欲和能量平衡可将这一风险因素降至最低。其中，瘦素和黑色素皮质素通路是维持食欲和能量平衡的关键机制：本研究旨在探讨胆石症患者体内 LEP、LEPR、TrkB、BDNF、POMC 和 MC4R 蛋白水平之间的关系。本研究旨在确定在维持食欲和能量平衡中发挥作用的 LEP、LEPR、TrkB、BDNF、POMC 和 MC4P 蛋白水平与胆石症之间的关系：本研究对 44 名确诊为胆石症的患者和 44 名先前未确诊为任何形式胆石症的健康对照组受试者进行了研究。采用酶联免疫吸附试验（ELISA）方法分析了瘦素（LEP）、瘦素结合瘦素受体（LEPR）、肌肽受体激酶 B（TrkB）、脑源性神经营养因子（BDNF）、前上皮细胞皮质素（POMC）和黑色素皮质素 4 受体（MC4R）分子的水平。结果采用 SPSS 软件（22.0 版）和 GraphPad Prism 8.0.1 软件进行分析：研究发现，与对照组相比，胆石症患者的 MC4R、TrkB、BDNF 和 POMC 蛋白水平明显下降（P < 0.05）。两组患者的LEP和LEPR浓度值差异无统计学意义（P=0.247，P=0.674）：结论：参与瘦素和黑色皮质素通路的蛋白质 MC4R、TrkB、BDNF 和 POMC 可能在胆石症疾病中发挥重要作用。不过，还需要对相关蛋白质进行更详细的研究。不过，这项研究将为新的研究提供指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Protein and Peptide Letters 生物-生化与分子生物学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Protein & Peptide Letters publishes letters, original research papers, mini-reviews and guest edited issues in all important aspects of protein and peptide research, including structural studies, advances in recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, and drug design. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallization and preliminary structure determination of biologically important proteins are considered only if they include significant new approaches or deal with proteins of immediate importance, and preliminary structure determinations of biologically important proteins. Purely theoretical/review papers should provide new insight into the principles of protein/peptide structure and function. Manuscripts describing computational work should include some experimental data to provide confirmation of the results of calculations. Protein & Peptide Letters focuses on: Structure Studies Advances in Recombinant Expression Drug Design Chemical Synthesis Function Pharmacology Enzymology Conformational Analysis Immunology Biotechnology Protein Engineering Protein Folding Sequencing Molecular Recognition Purification and Analysis