Retinoic acid modulates peripheral blood helper innate lymphoid cell composition in vitro in patients with multiple sclerosis

IF 2.9 4区医学 Q3 IMMUNOLOGY

Journal of neuroimmunology Pub Date : 2024-11-17 DOI:10.1016/j.jneuroim.2024.578489

Altuğ Özkoşar , Fatma Betül Öktelik , Metin Yusuf Gelmez , Sevda Öztürk Erden , Tuncay Gündüz , Murat Kürtüncü , Günnur Deniz , Suzan Çınar

引用次数: 0

Abstract

This study investigates the frequency and numbers of circulating helper innate lymphoid cells (ILCs) in untreated relapsing-remitting multiple sclerosis (RRMS) patients, focusing on intracellular IL-10 and CCR6 expressions under IL-2, IL-33, and retinoic acid (RA) stimulation in vitro and their associations with clinical features in RRMS. In RRMS patients, ILC1 levels were notably higher upon IL-2 + IL-33 + RA stimulation, while ILC2 levels, particularly the c-Kit⁺ ILC2 and CCR6⁺ ILC2 subsets, were significantly lower compared to unstimulated conditions. Additionally, IL-10⁺ ILC1 levels were elevated. The ratios of IL-10⁺ ILC1/ILC1, c-Kit⁺ ILC2/c-Kit⁻ ILC2, and CCR6⁺ ILC2/ILC2 were associated with the progression index (PI) in RRMS patients.

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维甲酸可调节多发性硬化症患者体外外周血辅助性先天性淋巴细胞的组成。

本研究调查了未经治疗的复发缓解型多发性硬化症（RRMS）患者体内循环辅助性先天性淋巴细胞（ILC）的频率和数量，重点研究了体外IL-2、IL-33和维甲酸（RA）刺激下细胞内IL-10和CCR6的表达及其与RRMS临床特征的关系。在RRMS患者中，IL-2+IL-33+RA刺激下ILC1水平明显升高，而ILC2水平，尤其是c-Kit+ ILC2和CCR6+ ILC2亚群，与非刺激条件下相比明显降低。此外，IL-10+ ILC1 水平升高。IL-10+ ILC1/ILC1、c-Kit+ ILC2/c-Kit- ILC2和CCR6+ ILC2/ILC2的比率与RRMS患者的病情进展指数（PI）相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of neuroimmunology 医学-免疫学

CiteScore

6.10

自引率

3.00%

发文量

154

审稿时长

37 days

期刊介绍： The Journal of Neuroimmunology affords a forum for the publication of works applying immunologic methodology to the furtherance of the neurological sciences. Studies on all branches of the neurosciences, particularly fundamental and applied neurobiology, neurology, neuropathology, neurochemistry, neurovirology, neuroendocrinology, neuromuscular research, neuropharmacology and psychology, which involve either immunologic methodology (e.g. immunocytochemistry) or fundamental immunology (e.g. antibody and lymphocyte assays), are considered for publication.