Curcumin-Loaded Long-Circulation Liposomes Ameliorate Insulin Resistance in Type 2 Diabetic Mice.

IF 6.6 2区 医学 Q1 NANOSCIENCE & NANOTECHNOLOGY
International Journal of Nanomedicine Pub Date : 2024-11-19 eCollection Date: 2024-01-01 DOI:10.2147/IJN.S487519
Kang-Xin Li, Hui Yuan, Jing Zhang, Xiao-Bin Peng, Wei-Fen Zhuang, Wen-Tao Huang, Hui-Xin Liang, Ying Lin, Ying-Zhen Huang, Shu-Lan Qin
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引用次数: 0

Abstract

Introduction: Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterised by insulin resistance, hyperglycaemia, and inflammation, with oxidative stress contributing to its progression. Curcumin (CUR), known for its anti-inflammatory, antioxidant, and insulin sensitising effects, has shown potential for the treatment of T2DM but is limited by low solubility and bioavailability. This study investigated long-circulating curcumin-loaded liposomes (CUR-LPs) to improve curcumin stability, solubility, and circulation and assessed their effect on insulin resistance in a murine model of T2DM.

Methods: CUR-LPs were prepared using the ethanol injection method and characterized for morphology, particle size, zeta potential, encapsulation efficiency, drug-loading capacity, and in vitro release. Cell viability was tested on murine L929 cells. In a T2DM murine model, after four weeks of CUR-LP treatment, inflammatory markers TNF-α and IL-6 were measured by real-time polymerase chain reaction, and liver tissues were analyzed for glutathione (GSH) and superoxide dismutase (SOD) via colorimetry.

Results: CUR-LPs were spherical, with an average diameter of (249 ± 2.3) nm and a zeta potential of (-33.5 ± 0.8) mV. They exhibited an encapsulation efficiency of (99.2 ± 0.5) %and a drug-loading capacity of (1.63 ± 0.02) %. CUR embedding in liposomes significantly maintained CUR release. In L929 cells, over 80% viability was maintained at 12 uM CUR concentration after 24 h. In HFD/STZ-induced T2DM mice, CUR-LPs improved blood glucose and insulin levels more efficiently than free CUR, and CUR-LPs also reduced hepatic inflammation (TNF-α, IL-6), enhanced hepatic GSH and SOD, and attenuated liver injury.

Conclusion: CUR-LPs improved glucose metabolism and insulin resistance in HFD/STZ-induced T2DM mice, which may be associated with a decrease in liver inflammation and oxidative stress.

姜黄素包裹的长循环脂质体可改善 2 型糖尿病小鼠的胰岛素抵抗。
简介2 型糖尿病(T2DM)是一种以胰岛素抵抗、高血糖和炎症为特征的代谢紊乱疾病,氧化应激是导致其恶化的原因之一。姜黄素(CUR)以其抗炎、抗氧化和胰岛素增敏作用而闻名,已显示出治疗 T2DM 的潜力,但由于溶解度和生物利用度较低而受到限制。本研究调查了长循环姜黄素负载脂质体(CUR-LPs),以改善姜黄素的稳定性、溶解性和循环,并评估其对小鼠 T2DM 模型中胰岛素抵抗的影响:方法:采用乙醇注射法制备 CUR-LPs,并对其形态、粒度、ZETA电位、包封效率、载药能力和体外释放进行表征。在小鼠 L929 细胞上测试了细胞活力。在 T2DM 小鼠模型中,CUR-LP 治疗四周后,通过实时聚合酶链反应测定了炎症标志物 TNF-α 和 IL-6,并通过比色法分析了肝组织中的谷胱甘肽(GSH)和超氧化物歧化酶(SOD):CUR-LPs呈球形,平均直径为(249 ± 2.3)纳米,zeta电位为(-33.5 ± 0.8)毫伏。它们的封装效率为 (99.2 ± 0.5) %,药物负载能力为 (1.63 ± 0.02) %。包埋在脂质体中的 CUR 能显著维持 CUR 的释放。在 HFD/STZ 诱导的 T2DM 小鼠中,CUR-LPs 比游离 CUR 更有效地改善了血糖和胰岛素水平,CUR-LPs 还降低了肝脏炎症(TNF-α、IL-6),增强了肝脏 GSH 和 SOD,减轻了肝损伤:结论:CUR-LPs能改善高纤维脂肪饮食/STZ诱导的T2DM小鼠的糖代谢和胰岛素抵抗,这可能与肝脏炎症和氧化应激的减少有关。
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来源期刊
International Journal of Nanomedicine
International Journal of Nanomedicine NANOSCIENCE & NANOTECHNOLOGY-PHARMACOLOGY & PHARMACY
CiteScore
14.40
自引率
3.80%
发文量
511
审稿时长
1.4 months
期刊介绍: The International Journal of Nanomedicine is a globally recognized journal that focuses on the applications of nanotechnology in the biomedical field. It is a peer-reviewed and open-access publication that covers diverse aspects of this rapidly evolving research area. With its strong emphasis on the clinical potential of nanoparticles in disease diagnostics, prevention, and treatment, the journal aims to showcase cutting-edge research and development in the field. Starting from now, the International Journal of Nanomedicine will not accept meta-analyses for publication.
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