Fecal fatty acid-linked bile acid profiles in pediatric patients with ulcerative colitis

IF 3.2 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinica Chimica Acta Pub Date : 2024-11-23 DOI:10.1016/j.cca.2024.120060

Hiromi Suzuki , Mitsuyoshi Suzuki , Keisuke Jimbo , Musashi Hibio , Takahiro Sasaki , Tsuyoshi Murai , Yukiko Yamashita , Mizuho Une , Shojiro Ogawa , Toru Okamoto , Seiko Narushima , Wataru Suda , Genta Kakiyama , Taka-aki Matsuyama , Hajime Takei , Hiroshi Nittono

{"title":"Fecal fatty acid-linked bile acid profiles in pediatric patients with ulcerative colitis","authors":"Hiromi Suzuki , Mitsuyoshi Suzuki , Keisuke Jimbo , Musashi Hibio , Takahiro Sasaki , Tsuyoshi Murai , Yukiko Yamashita , Mizuho Une , Shojiro Ogawa , Toru Okamoto , Seiko Narushima , Wataru Suda , Genta Kakiyama , Taka-aki Matsuyama , Hajime Takei , Hiroshi Nittono","doi":"10.1016/j.cca.2024.120060","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>Effect of gut dysbiosis on fatty acid (FA) linked 3β-hydroxy-bile acid esters (FA-isoBAs) formation is currently unknown. This study aimed to investigate the profile of FA-isoBAs in fecal samples from pediatric patients with ulcerative colitis (UC).</div></div><div><h3>Methods</h3><div>Fecal samples were collected from seven pediatric patients diagnosed with UC and seven age-matched healthy controls. Liquid chromatography-tandem mass spectrometry (LC/MS) method was set up for quantifications of thirteen different FA-isoBAs, including three new FA-isoBAs which had never been characterized. Method validation tests were performed with optimization of sample preparation. Statistical analyses were conducted to compare FA-isoBA concentrations between UC patients and healthy controls.</div></div><div><h3>Results</h3><div>The LC/MS method had sufficient linearity (r > 0.998), with the low detection limit (0.03–2.82 nmol/g stool), and limits of quantification (0.10–9.40 nmol/g stool) for all FA-isoBAs. The total FA-isoBAs concentration in UC patients was significantly lower than healthy controls, regardless of the degree of the disease severity. The UC subjects had markedly increased primary bile acid (BA) levels with decreased secondary BAs. Propionic acid-linked isoBA and stearic acid-linked 12-oxo-isolithocholic acid were newly identified from healthy subjects but they were not present in UC subjects. In agreement with the previous report, composition of long-chain (C16-C18) FA-linked BAs dominated over short-chain FA-linked BAs both in healthy and disease subjects.</div></div><div><h3>Conclusion</h3><div>The present study reported the fecal FA-isoBA profiles in pediatric UC patients for the first time. The results open the door to the new field that investigates the role of these microbial-driven BAs in gastrointestinal health.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"566 ","pages":"Article 120060"},"PeriodicalIF":3.2000,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinica Chimica Acta","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0009898124023131","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

Effect of gut dysbiosis on fatty acid (FA) linked 3β-hydroxy-bile acid esters (FA-isoBAs) formation is currently unknown. This study aimed to investigate the profile of FA-isoBAs in fecal samples from pediatric patients with ulcerative colitis (UC).

Methods

Fecal samples were collected from seven pediatric patients diagnosed with UC and seven age-matched healthy controls. Liquid chromatography-tandem mass spectrometry (LC/MS) method was set up for quantifications of thirteen different FA-isoBAs, including three new FA-isoBAs which had never been characterized. Method validation tests were performed with optimization of sample preparation. Statistical analyses were conducted to compare FA-isoBA concentrations between UC patients and healthy controls.

Results

The LC/MS method had sufficient linearity (r > 0.998), with the low detection limit (0.03–2.82 nmol/g stool), and limits of quantification (0.10–9.40 nmol/g stool) for all FA-isoBAs. The total FA-isoBAs concentration in UC patients was significantly lower than healthy controls, regardless of the degree of the disease severity. The UC subjects had markedly increased primary bile acid (BA) levels with decreased secondary BAs. Propionic acid-linked isoBA and stearic acid-linked 12-oxo-isolithocholic acid were newly identified from healthy subjects but they were not present in UC subjects. In agreement with the previous report, composition of long-chain (C16-C18) FA-linked BAs dominated over short-chain FA-linked BAs both in healthy and disease subjects.

Conclusion

The present study reported the fecal FA-isoBA profiles in pediatric UC patients for the first time. The results open the door to the new field that investigates the role of these microbial-driven BAs in gastrointestinal health.

查看原文本刊更多论文

溃疡性结肠炎儿科患者的粪便脂肪酸链胆汁酸谱。

目的：肠道菌群失调对与脂肪酸（FA）相连的3β-羟基胆酸酯（FA-isoBAs）形成的影响目前尚不清楚。本研究旨在调查溃疡性结肠炎（UC）儿科患者粪便样本中 FA-isoBAs 的概况：方法：从七名确诊为溃疡性结肠炎的儿科患者和七名年龄匹配的健康对照者中采集粪便样本。建立了液相色谱-串联质谱（LC/MS）方法，用于定量检测13种不同的FA-isoBAs，包括3种从未定性的新FA-isoBAs。通过优化样品制备进行了方法验证测试。对 UC 患者和健康对照组的 FA-isoBA 浓度进行了统计分析：LC/MS方法具有良好的线性关系（r > 0.998），所有FA-isoBAs的检测限（0.03-2.82 nmol/g粪便）和定量限（0.10-9.40 nmol/g粪便）均较低。无论疾病严重程度如何，UC 患者的 FA-isoBAs 总浓度都明显低于健康对照组。UC 受试者的一级胆汁酸 (BA) 水平明显升高，而二级胆汁酸则有所下降。从健康受试者中新发现了丙酸连接的异胆汁酸和硬脂酸连接的 12-氧代异胆汁酸，但它们在 UC 受试者中并不存在。与之前的报告一致，在健康受试者和疾病受试者中，长链（C16-C18）FA-连接的BA的组成均以短链FA-连接的BA为主：结论：本研究首次报道了小儿 UC 患者粪便中 FA-isoBA 的概况。结论：本研究首次报道了小儿 UC 患者粪便中的 FA-isoBA 特征，其结果为研究这些微生物驱动的 BA 在胃肠道健康中的作用打开了一扇新的大门。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Clinica Chimica Acta 医学-医学实验技术

CiteScore

10.10

自引率

2.00%

发文量

1268

审稿时长

23 days

期刊介绍： The Official Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Clinica Chimica Acta is a high-quality journal which publishes original Research Communications in the field of clinical chemistry and laboratory medicine, defined as the diagnostic application of chemistry, biochemistry, immunochemistry, biochemical aspects of hematology, toxicology, and molecular biology to the study of human disease in body fluids and cells. The objective of the journal is to publish novel information leading to a better understanding of biological mechanisms of human diseases, their prevention, diagnosis, and patient management. Reports of an applied clinical character are also welcome. Papers concerned with normal metabolic processes or with constituents of normal cells or body fluids, such as reports of experimental or clinical studies in animals, are only considered when they are clearly and directly relevant to human disease. Evaluation of commercial products have a low priority for publication, unless they are novel or represent a technological breakthrough. Studies dealing with effects of drugs and natural products and studies dealing with the redox status in various diseases are not within the journal''s scope. Development and evaluation of novel analytical methodologies where applicable to diagnostic clinical chemistry and laboratory medicine, including point-of-care testing, and topics on laboratory management and informatics will also be considered. Studies focused on emerging diagnostic technologies and (big) data analysis procedures including digitalization, mobile Health, and artificial Intelligence applied to Laboratory Medicine are also of interest.