Multi-omics Analysis of Klebsiella pneumoniae Revealed Opposing Effects of Rutin and Luteolin on Strain Growth.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2024-11-25 DOI:10.1007/s00284-024-03982-5

Zhibin Wang, Wanxia Shen, Yuejiao Li, Xiaoyun Wang, Xiaolin Zhong, Xing Wang

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Abstract

The emergence of pathogenic bacteria resistant to conventional antibiotics is becoming increasingly challenging. Plant-derived flavonoids are potential alternatives to antibiotics, owing to their antimicrobial properties. However, the molecular mechanisms through which they inhibit the growth of pathogenic microorganisms remain unclear. Therefore, Klebsiella pneumoniae ATCC700603 was separately incubated in two flavonoids to elucidate their inhibitory mechanism. Metabolomic and transcriptomic analyses were performed after 4-h incubation. In total, 5483 genes and 882 metabolites were identified. Compared to the untreated control, rutin and luteolin activated 507 and 374 differentially expressed genes (DEGs), respectively. However, the number of differential abundant metabolites (DAMs) remained the same. The top 10 correlated DEGs and DAMs were identified within each comparative group after a correlation analysis. Rutin induced the accumulation of unique metabolites and suppressed gene expression whereas luteolin did not. Our results explain the disparate effects of these two flavonoids and demonstrate the inhibitory mechanism of rutin on strain growth.

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肺炎克雷伯氏菌的多组学分析揭示了芦丁和木犀草素对菌株生长的相反影响

对传统抗生素产生抗药性的病原菌的出现正变得越来越具有挑战性。植物黄酮类化合物具有抗菌特性，是抗生素的潜在替代品。然而，它们抑制病原微生物生长的分子机制仍不清楚。因此，肺炎克雷伯氏菌 ATCC700603 分别与两种黄酮类化合物进行培养，以阐明它们的抑制机制。培养 4 小时后进行了代谢组和转录组分析。共鉴定出 5483 个基因和 882 种代谢物。与未处理的对照组相比，芦丁和木犀草素分别激活了 507 和 374 个差异表达基因（DEGs）。然而，差异丰度代谢物（DAMs）的数量保持不变。经过相关性分析，在每个比较组中确定了前 10 个相关的 DEGs 和 DAMs。芦丁诱导了独特代谢物的积累并抑制了基因表达，而叶黄素则没有。我们的研究结果解释了这两种黄酮类化合物的不同作用，并证明了芦丁对菌株生长的抑制机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.