Granular Nanofiber-Hydrogel Composite-Programmed Regenerative Inflammation and Adipose Tissue Formation.

IF 10 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Jiayuan Kong, Zhi-Cheng Yao, Jessica L Stelzel, Yueh-Hsun Yang, Jeffrey Chen, Hexiang Feng, Collin Schmidt, Chi Zhang, Kedar Krishnan, Long Chen, Jingwen Pan, Kailei Ding, Yining Zhu, Xiaowei Li, Joshua C Doloff, Hai-Quan Mao, Sashank K Reddy
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Abstract

The interplay between biomaterials and host immune responses critically determines outcomes in tissue restoration. Recent studies suggest that physicochemical properties of materials can dictate pro-regenerative versus pro-fibrotic responses and have begun to define the key immune cell types and signals governing these divergent effects. This emerging understanding enables the engineering of regenerative biomaterials capable of functional restoration in situ. An injectable nanofiber-hydrogel composite (NHC) microparticles are designed and constructed from cross-linked electrospun collagen nanofiber fragments surface-bonded to the hyaluronic acid hydrogel network via covalent conjugation during the cross-linking process. The collagen nanofiber fragments, acting as the structural reinforcement component, increased the overall storage modulus of the NHC to a level comparable to native soft tissues while maintaining a sufficiently high degree of porosity of the hydrogel phase to allow host cell infiltration following subcutaneous injection of the NHC microparticles. More importantly, the NHC promoted macrophage/monocyte infiltration, migration, and spreading, sustained cell recruitment over time, and enhanced the proangiogenic effect and recruitment of PDGFRα+ perivascular progenitor cells, leading to extensive adipose tissue remodeling. This study demonstrates the regenerative potential of the injectable NHC microgels as an off-the-shelf solution for devastating soft tissue losses.

颗粒状纳米纤维-水凝胶复合材料编程再生炎症和脂肪组织的形成
生物材料与宿主免疫反应之间的相互作用对组织修复的结果起着至关重要的决定作用。最近的研究表明,材料的物理化学特性可决定促进再生和促进纤维化的反应,并已开始确定支配这些不同效应的关键免疫细胞类型和信号。这种新的认识使得再生生物材料的工程设计能够就地恢复功能。我们设计了一种可注射的纳米纤维-水凝胶复合材料(NHC)微颗粒,它是由交联电纺胶原纳米纤维片段通过交联过程中的共价键合作用与透明质酸水凝胶网络表面结合而成。作为结构加固成分的胶原纳米纤维片段将 NHC 的整体储存模量提高到了与原生软组织相当的水平,同时保持了水凝胶相足够高的孔隙度,使宿主细胞在皮下注射 NHC 微颗粒后能够浸润。更重要的是,NHC能促进巨噬细胞/单核细胞的浸润、迁移和扩散,使细胞招募持续一段时间,并增强促血管生成效应和PDGFRα+血管周围祖细胞的招募,从而导致脂肪组织的广泛重塑。这项研究证明了可注射 NHC 微凝胶的再生潜力,它是治疗破坏性软组织损失的现成解决方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advanced Healthcare Materials
Advanced Healthcare Materials 工程技术-生物材料
CiteScore
14.40
自引率
3.00%
发文量
600
审稿时长
1.8 months
期刊介绍: Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.
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