A comparison study on compounding oat β-glucan onto Pleurotus eryngii protein by using different methods: In vitro digestion and fermentation characteristics

IF 11 1区 农林科学 Q1 CHEMISTRY, APPLIED
Qi Tao, Xinyi Li, Sai Ma, Qiuhui Hu, Gaoxing Ma
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Abstract

This study aimed to investigate the effects under four different composite methods (electrostatic self-assembly, ultrasonic cavitation, high-pressure homogenization, and wet glycosylation), oat β-glucan (OG) on the in vitro digestion properties and in vitro fermentation properties of Pleurotus eryngii protein (PEP). The changes in the degree of protein dissociations and structures during digestion, pH and short-chain fatty acids (SCFAs) during fermentation, and the structures of intestinal flora at the end of fermentation were evaluated. The results showed that during in vitro digestion, the complexes could inhibit the degree of dissociation of PEP. During in vitro fermentation, several complexes enhanced the production of SCFAs and promoted the proliferation of beneficial bacteria compared with PEP and OG. Moreover, the complexes obtained by wet glycosylation showed a better effect in inhibiting the dissociation degree of PEP, increasing the production of SCFAs, and enhancing the abundance of beneficial bacteria. Hence, studying the impacts of different compounding methods on their in vitro digestion and fermentation properties, helps us to prepare functional synthetic ingredients with more probiotic potential.

Abstract Image

用不同方法将燕麦β-葡聚糖复合到红豆杉蛋白上的比较研究:体外消化和发酵特性
本研究旨在探讨在四种不同的复合方法(静电自组装、超声波空化、高压均质化和湿糖基化)下,燕麦β-葡聚糖(OG)对红豆杉蛋白质(PEP)的体外消化特性和体外发酵特性的影响。研究评估了消化过程中蛋白质解离程度和结构的变化、发酵过程中pH值和短链脂肪酸(SCFAs)的变化以及发酵结束时肠道菌群结构的变化。结果表明,在体外消化过程中,复合物能抑制 PEP 的解离程度。在体外发酵过程中,与 PEP 和 OG 相比,几种复合物能提高 SCFAs 的产生,促进有益菌的增殖。此外,湿糖基化得到的复合物在抑制 PEP 的解离度、增加 SCFAs 的产生和提高有益菌的数量方面表现出更好的效果。因此,研究不同复配方法对其体外消化和发酵特性的影响,有助于我们制备出更具益生潜力的功能性合成配料。
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来源期刊
Food Hydrocolloids
Food Hydrocolloids 工程技术-食品科技
CiteScore
19.90
自引率
14.00%
发文量
871
审稿时长
37 days
期刊介绍: Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication. The main areas of interest are: -Chemical and physicochemical characterisation Thermal properties including glass transitions and conformational changes- Rheological properties including viscosity, viscoelastic properties and gelation behaviour- The influence on organoleptic properties- Interfacial properties including stabilisation of dispersions, emulsions and foams- Film forming properties with application to edible films and active packaging- Encapsulation and controlled release of active compounds- The influence on health including their role as dietary fibre- Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes- New hydrocolloids and hydrocolloid sources of commercial potential. The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.
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