Chitosan obtained from black soldier fly larval cuticles expands the value chain and is effective as a biocontrol agent to combat plant pathogens

IF 10.7 1区 化学 Q1 CHEMISTRY, APPLIED
Carolina Escobar Rodríguez , Valeriia Zaremska , Thomas Klammsteiner , Ioannis Kampatsikas , Nils Münstermann , Oliver Weichold , Sabine Gruber
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Abstract

The industrial use of certain insects, such as the black soldier fly (BSF, Hermetia illucens), has become a burgeoning way of converting residual biomass into a valuable source of biomolecules for the feed and food industry. Here, the integration of a valuable step as an upcycling technology using biological waste from the 5th instar BSF larvae processing as a source of bioactive chitosan was explored. The goal is to pave the way for sustainable chitosan production from insect-based resources in addition to proteins, lipids, and fertilizers. The macronutrient profile of the larval waste was evaluated for chitosan extraction. After homogenization and mechanical separation of the larval pulp, enzyme-assisted extraction of chitosan from the chitin-rich matrix was performed using different combinations of commercially available proteases and lipases, followed by chemical deacetylation. The antifungal efficacy of the recovered chitosan varied depending on the extracted product and tested fungal plant pathogens. Furthermore, the plant priming effect of chitosan on Beta vulgaris promoted plant vigor and disease resistance. Seed coating resulted in improved germination and primary root growth. Thus, the extraction of chitosan from BSF larval cuticles offers an optimistic outlook for expanding the technological competence of the insect upcycling industries.

Abstract Image

从黑实蝇幼虫角质层中提取的壳聚糖拓展了价值链,可作为生物控制剂有效对抗植物病原体
对某些昆虫(如黑兵蝇(BSF,Hermetia illucens))的工业利用已成为将残余生物质转化为饲料和食品行业宝贵的生物大分子来源的一种新兴方式。在此,研究人员探索了如何利用 BSF 5龄幼虫加工产生的生物废物作为生物活性壳聚糖的来源,将这一有价值的步骤整合为升级再循环技术。其目的是在蛋白质、脂类和肥料之外,为利用昆虫资源生产可持续壳聚糖铺平道路。为提取壳聚糖,对幼虫废弃物的宏量营养成分进行了评估。在对幼虫果肉进行均质化和机械分离后,使用市售蛋白酶和脂肪酶的不同组合从富含甲壳素的基质中进行酶辅助提取壳聚糖,然后进行化学脱乙酰处理。回收壳聚糖的抗真菌功效因提取产品和测试的植物真菌病原体而异。此外,壳聚糖对 Beta vulgaris 的植物引诱作用促进了植物的活力和抗病性。种子包衣提高了发芽率和主根生长。因此,从 BSF 幼虫角质层中提取壳聚糖为扩大昆虫升级再造产业的技术能力提供了乐观的前景。
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来源期刊
Carbohydrate Polymers
Carbohydrate Polymers 化学-高分子科学
CiteScore
22.40
自引率
8.00%
发文量
1286
审稿时长
47 days
期刊介绍: Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.
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