Comparing enzymatic post-treatments by endoglucanase (EG) and lytic polysaccharide monooxygenase (LPMO) on microfibrillated cellulose (MFC) to enhance cellulose film fabrication

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2024-11-18 DOI:10.1016/j.carbpol.2024.123037

Jie Wu , Yufeng Yuan , Qi Hua , Tao Zou , Zhangmin Wan , Gio Ferson Bautista , Orlando Rojas , Scott Renneckar , Jack Saddler

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Abstract

Cellulose is the world's most abundant natural polymer and it can be used as a substitute for fossil derived products. The work described here evaluated the use of mono-component enzyme treatment, using endoglucanase (EG) and lytic polysaccharide monooxygenase (LPMO), to improve the properties of micro-fibrillated cellulose (MFC) produced from mechanically refined kraft pulp. Endoglucanase treatment of the pulp significantly reduced the degree of polymerization (DP) of the cellulose by promoting fiber cutting. In contrast, LPMO treatment only slightly reduced the fiber length and pulp viscosity. However, the introduction of carboxylic acid groups by the action of LPMO appeared to enhance the accessible surface area of cellulose. Enzyme-treated MFC showed improved nano-fibrillation with transmittance and water retention values increased after both enzyme treatments. The cellulose films derived from enzyme-treated MFC showed enhanced mechanical properties with the LPMO-treated films showing superior tensile strength (77 MPa) and Young's modulus (3600 MPa). Thermogravimetric analysis (TGA) also indicated improved thermal stability for both enzyme-treated films. LPMO treatments proved more effective, promoting nano-fibrillation while maintaining fiber integrity and enhanced intermolecular interactions.

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比较内切葡聚糖酶（EG）和裂解多糖单氧化酶（LPMO）对微纤化纤维素（MFC）的酶后处理，以提高纤维素膜的制造水平

纤维素是世界上最丰富的天然聚合物，可用作化石衍生产品的替代品。本文介绍的工作评估了使用内切葡聚糖酶（EG）和裂解多糖单氧酶（LPMO）进行单组分酶处理，以改善从机械精制牛皮浆生产的微纤化纤维素（MFC）的特性。对纸浆进行内切葡聚糖酶处理可促进纤维切割，从而显著降低纤维素的聚合度（DP）。相比之下，LPMO 处理只能轻微降低纤维长度和纸浆粘度。不过，在 LPMO 作用下引入的羧酸基团似乎增加了纤维素的可触及表面积。经酶处理的 MFC 在两种酶处理后都显示出纳米纤维化的改善，透射率和保水值都有所提高。经酶处理的 MFC 纤维素薄膜显示出更强的机械性能，经 LPMO 处理的薄膜显示出更高的拉伸强度（77 兆帕）和杨氏模量（3600 兆帕）。热重分析（TGA）也表明，两种酶处理薄膜的热稳定性都有所提高。事实证明，LPMO 处理更有效，在保持纤维完整性和增强分子间相互作用的同时促进了纳米纤维化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.