Functional Au@Cu/Cu2O/C composites derived from 2D Cu-based metal–organic framework for sensitive label-free electrochemical immunoassay

IF 4.1 3区化学 Q1 CHEMISTRY, ANALYTICAL

Journal of Electroanalytical Chemistry Pub Date : 2024-11-19 DOI:10.1016/j.jelechem.2024.118815

Baojun Han , Shuqin Fang , Yu Wang, Jiajun Ma, Jinling Wu, Xiaoming Ma, Qingchun Lan

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引用次数: 0

Abstract

Metal-organic frameworks (MOFs) face challenges in electrochemical sensing due to low conductivity and poor stability. Herein, we report the synthesis of MOFs-derived metal/carbon composites (Au@Cu/Cu₂O/C) that exhibit synergistic effects by combining metallic and carbonaceous characteristics due to their hierarchical structures and metal contents. The Au@Cu/Cu₂O/C composites were characterized using electron microscopy, X-ray diffraction, Raman spectroscopy, and X-ray photoelectron spectroscopy. These composites inherit the exceptional properties of the pristine MOFs while exhibiting a large specific surface area, high electrical conductivity and stability. As an excellent electrode material, the Au@Cu/Cu₂O/C composites were biofunctionalized with streptavidin and a biotinylated antibody for use as a highly sensitive, label-free electrochemical immunosensor for the detection of the tumor marker prostate specific antigen (PSA). The fabricated immunosensor successfully detected PSA concentrations ranging from 0.05 to 60 ng/mL, with a detection limit of 0.01 ng/mL, showcasing high sensitivity comparable to other existing methods. This research provides a versatile and promising platform for developing advanced biosensors for disease diagnostics and further expanding the application of MOFs.

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源自二维铜基金属有机框架的功能性 Au@Cu/Cu2O/C 复合材料用于灵敏的无标记电化学免疫分析

金属有机框架（MOFs）由于电导率低、稳定性差，在电化学传感领域面临挑战。在此，我们报告了由 MOFs 衍生的金属/碳复合材料（Au@Cu/Cu2O/C）的合成过程，由于其分层结构和金属含量，这些复合材料结合了金属和碳的特性，从而表现出协同效应。利用电子显微镜、X 射线衍射、拉曼光谱和 X 射线光电子能谱对 Au@Cu/Cu2O/C 复合材料进行了表征。这些复合材料继承了原始 MOFs 的优异特性，同时具有大比表面积、高导电性和稳定性。作为一种优良的电极材料，Au@Cu/Cu2O/C 复合材料与链霉亲和素和生物素化抗体进行了生物功能化，可用作一种高灵敏度、无标记的电化学免疫传感器，用于检测肿瘤标志物前列腺特异性抗原（PSA）。制备的免疫传感器成功检测了 0.05 至 60 纳克/毫升的前列腺特异性抗原浓度，检测限为 0.01 纳克/毫升，显示出与其他现有方法相当的高灵敏度。这项研究为开发用于疾病诊断的先进生物传感器提供了一个多功能且前景广阔的平台，并进一步拓展了 MOFs 的应用领域。

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来源期刊

Journal of Electroanalytical Chemistry 化学-电化学

CiteScore

7.80

自引率

6.70%

发文量

912

审稿时长

2.4 months

期刊介绍： The Journal of Electroanalytical Chemistry is the foremost international journal devoted to the interdisciplinary subject of electrochemistry in all its aspects, theoretical as well as applied. Electrochemistry is a wide ranging area that is in a state of continuous evolution. Rather than compiling a long list of topics covered by the Journal, the editors would like to draw particular attention to the key issues of novelty, topicality and quality. Papers should present new and interesting electrochemical science in a way that is accessible to the reader. The presentation and discussion should be at a level that is consistent with the international status of the Journal. Reports describing the application of well-established techniques to problems that are essentially technical will not be accepted. Similarly, papers that report observations but fail to provide adequate interpretation will be rejected by the Editors. Papers dealing with technical electrochemistry should be submitted to other specialist journals unless the authors can show that their work provides substantially new insights into electrochemical processes.