Exopolysaccharides from Bifidobacterium longum subsp. infantis and Bifidobacterium adolescentis modulate Toll-like receptor signaling

IF 10.7 1区 化学 Q1 CHEMISTRY, APPLIED
Renate Akkerman , Marjolein M.P. Oerlemans , Michela Ferrari , Cynthia Fernández-Lainez , Marthe T.C. Walvoort , Paul de Vos
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Abstract

Background

Exopolysaccharides (EPS) from probiotic bacteria like bifidobacteria, have gained considerable attention for the beneficial effects they exert in the gastrointestinal environment. Here, we investigated whether EPS isolated from Bifidobacterium longum subsp. infantis and Bifidobacterium adolescentis can interact with Toll-like receptors (TLRs) in a structure-dependent way and subsequently we investigated whether they influence cytokine-production in dendritic cells (DCs).

Results

EPS from both B. infantis and B. adolescentis were found to be structurally different and were able to inhibit signaling of TLR2 and TLR4 in an EPS-type dependent fashion. EPS from B. infantis was shown to have stronger inhibitory effects on TLR2/1, whereas EPS from B. adolescentis showed stronger effects for TLR2/6 and TLR4. Incubation of DCs with EPS alone had no effect, however stimulation of DCs with spend-medium of epithelial cells incubated with EPS reduced production of the cytokines MCP-1/CCL2 and TNFα.

Conclusion

Here we show that EPS from B. infantis and B. adolescentis have structure-dependent immunomodulatory effects, indicating that EPS might be important effector molecules responsible for the health benefits of bifidobacteria.

Abstract Image

长双歧杆菌亚种和青春期双歧杆菌的外多糖调节Toll样受体信号转导
背景来自双歧杆菌等益生菌的多糖(EPS)因其在胃肠道环境中发挥的有益作用而备受关注。在此,我们研究了从长双歧杆菌婴儿亚种和青春期双歧杆菌中分离出来的 EPS 是否能以结构依赖的方式与 Toll 样受体(TLRs)相互作用,并随后研究了它们是否会影响树突状细胞(DCs)中细胞因子的产生。结果发现婴儿双歧杆菌和青春期双歧杆菌的 EPS 在结构上有所不同,并能以 EPS 类型依赖的方式抑制 TLR2 和 TLR4 的信号转导。研究表明,婴儿鹅膏菌的 EPS 对 TLR2/1 有更强的抑制作用,而青春期鹅膏菌的 EPS 对 TLR2/6 和 TLR4 有更强的抑制作用。仅用 EPS 培养直流细胞没有效果,但用 EPS 培养的上皮细胞的花费介质刺激直流细胞可减少细胞因子 MCP-1/CCL2 和 TNFα 的产生。
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来源期刊
Carbohydrate Polymers
Carbohydrate Polymers 化学-高分子科学
CiteScore
22.40
自引率
8.00%
发文量
1286
审稿时长
47 days
期刊介绍: Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.
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