Modulating hydrophobic and antimicrobial gelatin-zein protein-based bilayer films by incorporating glycerol monolaurate and TiO2 nanoparticles

IF 8.5 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Packaging and Shelf Life Pub Date : 2024-11-25 DOI:10.1016/j.fpsl.2024.101393

Md. Easdani , Fei Liu , Jan F.M. Van Impe , Shabbir Ahammed , Maoshen Chen , Fang Zhong

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引用次数: 0

Abstract

The hydrophobicity was developed on gelatin-zein bilayer films with enhanced antimicrobial properties and persistent inherent mechanical characterization by incorporating Glycerol monolaurate (GML) and nano-TiO₂. Atomic force microscopy (AFM) and field emission scanning electron microscopy (FESEM) revealed that GML exhibited roughness and reduced porosity on outer zein surfaces, resulting in a water contact angle (WCA) > 90°. Substantial GML migration was also observed from gelatin film to acetic acid-containing zein layers and improved hydrophobicity. Conversely, nano-TiO₂-assisted hydrophilic porous microstructure indicated lower WCA, and nano-TiO₂ influenced the regulation of mechanical strength near controlled TS ( ≈ 20 MPa) with improved thermal resistance, whereas the extended flexibility (elongation >200 %) performed through GML incorporation. There were slight variations in water solubility (% WS) and moisture content (% MC), with 1 % (w/v) GML signifying better results in reducing water vapor permeability (WVP) and oxygen permeability (OP). Differential scanning calorimetry (DSC), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR) analysis also presented GML migration, altering crystalline structures and protein conformation. Using the bacterial inactivation model for the liquid medium, including the log-linear inactivation phase and tail, Gram-positive S. aureus ATCC29213 reached a maximum of around 2 log reduction, but Gram-negative E. coli JM109 showed higher resistance. Along with the surface-mediated effect, GML-incorporated gelatin layers and migrated GML also exhibited inactivation efficacy for bacterial exposure on the outer surface for a considerable time. Hence, GML and nano-TiO₂ synergistically hold promise for bioactive packaging with significant applications.

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通过加入单月桂酸甘油酯和 TiO2 纳米颗粒，调节基于明胶-玉米蛋白的疏水抗菌双层膜

通过加入单月桂酸甘油酯（GML）和纳米二氧化钛，在明胶-玉米蛋白双层膜上开发了疏水性，增强了抗菌性能和持久的固有机械特性。原子力显微镜（AFM）和场发射扫描电子显微镜（FESEM）显示，甘油单月桂酸酯（GML）在玉米蛋白外表面表现出粗糙度并降低了孔隙率，导致水接触角（WCA）达到 90°。还观察到大量 GML 从明胶膜迁移到含醋酸的玉米蛋白层，并改善了疏水性。相反，纳米二氧化钛辅助的亲水多孔微结构显示出较低的 WCA，纳米二氧化钛影响了受控 TS 附近（≈ 20 兆帕）机械强度的调节，并改善了耐热性，而通过 GML 的加入则实现了柔韧性的延长（伸长率 > 200 %）。水溶性（WS%）和含水量（MC%）略有不同，1%（w/v）的 GML 在降低水蒸气渗透性（WVP）和氧气渗透性（OP）方面有更好的效果。差示扫描量热法（DSC）、X 射线衍射（XRD）和傅立叶变换红外光谱（FTIR）分析也显示了 GML 的迁移，改变了晶体结构和蛋白质构象。利用液体培养基的细菌灭活模型（包括对数线性灭活阶段和尾部），革兰氏阳性金黄色葡萄球菌 ATCC29213 的最大灭活率约为 2 对数，但革兰氏阴性大肠杆菌 JM109 的抗性更高。除了表面介导效应外，GML 内含明胶层和迁移的 GML 对外表面的细菌暴露时间也有灭活效果。因此，GML 和纳米二氧化钛的协同作用有望成为生物活性包装的重要应用。

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来源期刊

Food Packaging and Shelf Life Agricultural and Biological Sciences-Food Science

CiteScore

14.00

自引率

8.80%

发文量

214

审稿时长

70 days

期刊介绍： Food packaging is crucial for preserving food integrity throughout the distribution chain. It safeguards against contamination by physical, chemical, and biological agents, ensuring the safety and quality of processed foods. The evolution of novel food packaging, including modified atmosphere and active packaging, has extended shelf life, enhancing convenience for consumers. Shelf life, the duration a perishable item remains suitable for sale, use, or consumption, is intricately linked with food packaging, emphasizing its role in maintaining product quality and safety.