PpHsfA2a-regulated the accumulation of proline and polyamines participates in glycine betaine-enhanced chilling resistance in peach fruit

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2024-11-19 DOI:10.1016/j.postharvbio.2024.113320

Qingyuan Song , Li Wang , Yanyan Wang , Kaili Shi , Tingyu Wu , Tian Qiu , Jingjing Jiang , Xingyue Wang , Zhikang Liu , Peng Jin , Yonghua Zheng , Dan Chen

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引用次数: 0

Abstract

Application of glycine betaine (GB) was found to efficiently alleviate chilling injury (CI) of peach fruit under chilling conditions, but the molecular mechanism of which remains unclear. In this work, GB treatment retained lower internal browning index accompanied by lower relative conductivity and malondialdehyde content in cold stored peaches. Meanwhile, GB treatment elevated the gene transcription and activities of Δ ¹-pyroline-5-carboxylate synthetase (P5CS), ornithine δ-aminotransferase (OAT), arginase (ARG), arginine decarboxylase (ADC) and ornithine decarboxylase (ODC), weakened the gene transcription and activity of proline dehydrogenase (PDH), contributing to the accumulation of proline and polyamines (PAs). Moreover, PpHsfA2a was highly expressed under cold stress and GB treatment, which was further found to be located in the nucleus with transactivation capacity. Importantly, PpHsfA2a activated the expression of PpARG, PpOAT and PpADC by directly recognizing and binding to the HSE elements in their promoters. Consequently, these results suggested that PpHsfA2a-mediated the transcriptional activation of proline and PAs accumulation related genes was involved in GB-enhanced chilling resistance in peach fruit, which was beneficial for the integrity of cell membrane function and structure under cold conditions.

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PpHsfA2a-regulated the accumulation of proline and polyamines participate in glycine betaine-enhanced chilling resistance in peach fruit

研究发现，应用甘氨酸甜菜碱（GB）可有效缓解冷藏条件下桃果实的冷害（CI），但其分子机制仍不清楚。在这项研究中，GB 处理可降低冷藏桃的内部褐变指数，同时降低相对电导率和丙二醛含量。同时，GB 处理提高了Δ 1-脯氨酸-5-羧酸合成酶（P5CS）、鸟氨酸δ-氨基转移酶（OAT）、精氨酸酶（ARG）、精氨酸脱羧酶（ADC）和鸟氨酸脱羧酶（ODC）的基因转录和活性，削弱了脯氨酸脱氢酶（PDH）的基因转录和活性，促进了脯氨酸和多胺（PAs）的积累。此外，在冷胁迫和 GB 处理下，PpHsfA2a 高表达，并进一步发现其位于细胞核中，具有转录激活能力。重要的是，PpHsfA2a 通过直接识别并结合启动子中的 HSE 元件，激活了 PpARG、PpOAT 和 PpADC 的表达。因此，这些结果表明，PpHsfA2a 介导的脯氨酸和 PAs 积累相关基因的转录激活参与了 GB 增强桃果实抗寒性的过程，这有利于细胞膜功能和结构在低温条件下的完整性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.