Gut microbiota and intestinal FXR siganlling involved in the alleviation of mulberry (Morus alba L.) leaf ethanol extract on type 2 diabetes mellitus in db/db mice

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2024-11-23 DOI:10.1016/j.jff.2024.106600

Ding-xiang Li , Sheng-qiu Xu , Han Jiang , Ya-Ting Li , Yan-lin Zhao , Peng Jin , Shuai Ji , Yan Du , Dao-Quan Tang

{"title":"Gut microbiota and intestinal FXR siganlling involved in the alleviation of mulberry (Morus alba L.) leaf ethanol extract on type 2 diabetes mellitus in db/db mice","authors":"Ding-xiang Li , Sheng-qiu Xu , Han Jiang , Ya-Ting Li , Yan-lin Zhao , Peng Jin , Shuai Ji , Yan Du , Dao-Quan Tang","doi":"10.1016/j.jff.2024.106600","DOIUrl":null,"url":null,"abstract":"<div><div>The potential mechanism of mulberry leaf against type 2 diabetes mellitus (T2DM) is not fully elucidated. Here, we hypothesized that the anti-T2DM of mulberry leaf ethanol extract (MLEE) is related to improvement of gut microbiota and bile acids (BAs) metabolism. Our results showed that MLEE alone can ameliorate glycolipid metabolism disorder, insulin resistance, and inflammation in <em>db/db</em> mice, and presents synergistic effects with metformin (MET). Furthermore, we found that MLEE altered the abundances of gut microbiota related to BAs metabolism such as <em>Colidextribacter</em>, <em>Muribaculum</em>, <em>Muribaculaceae</em>, and <em>Eubacterium_siraeum_group</em>, promoted fecal ωMCA level and thus inhibited intestinal farnesoid X receptor (FXR) gene expression, and then increased plasma glucagon-like peptide-1 (GLP-1) level. Furthermore, intestinal FXR inhibition and plasma GLP-1 level enhancing of MLEE could also be observed in antibiotic-intervened <em>db/db</em> mice. These results indicated that MLEE could alleviate T2DM via gut microbiota-BAs-intestinal FXR pathway mediated by gut microbiota and host.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"123 ","pages":"Article 106600"},"PeriodicalIF":3.8000,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Functional Foods","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1756464624006030","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The potential mechanism of mulberry leaf against type 2 diabetes mellitus (T2DM) is not fully elucidated. Here, we hypothesized that the anti-T2DM of mulberry leaf ethanol extract (MLEE) is related to improvement of gut microbiota and bile acids (BAs) metabolism. Our results showed that MLEE alone can ameliorate glycolipid metabolism disorder, insulin resistance, and inflammation in db/db mice, and presents synergistic effects with metformin (MET). Furthermore, we found that MLEE altered the abundances of gut microbiota related to BAs metabolism such as Colidextribacter, Muribaculum, Muribaculaceae, and Eubacterium_siraeum_group, promoted fecal ωMCA level and thus inhibited intestinal farnesoid X receptor (FXR) gene expression, and then increased plasma glucagon-like peptide-1 (GLP-1) level. Furthermore, intestinal FXR inhibition and plasma GLP-1 level enhancing of MLEE could also be observed in antibiotic-intervened db/db mice. These results indicated that MLEE could alleviate T2DM via gut microbiota-BAs-intestinal FXR pathway mediated by gut microbiota and host.

Abstract Image

查看原文本刊更多论文

肠道微生物群和肠道 FXR siganlling 参与桑叶乙醇提取物对 db/db 小鼠 2 型糖尿病的缓解作用

桑叶抗2型糖尿病（T2DM）的潜在机制尚未完全阐明。在此，我们假设桑叶乙醇提取物（MLEE）的抗T2DM作用与肠道微生物群和胆汁酸（BAs）代谢的改善有关。我们的研究结果表明，单独使用桑叶乙醇提取物可以改善db/db小鼠的糖脂代谢紊乱、胰岛素抵抗和炎症，并与二甲双胍（MET）协同作用。此外，我们还发现，MLEE改变了与BAs代谢相关的肠道微生物群（如Colidextribacter、Muribaculum、Muribaculaceae和Eubacterium_siraeum_group）的丰度，提高了粪便中ωMCA的水平，从而抑制了肠道珐琅质X受体（FXR）基因的表达，进而提高了血浆胰高血糖素样肽-1（GLP-1）的水平。此外，在抗生素干预的db/db小鼠体内也可观察到MLEE抑制肠道FXR和提高血浆GLP-1水平的作用。这些结果表明，MLEE可通过由肠道微生物群和宿主介导的肠道微生物群-BA-肠道FXR途径缓解T2DM。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.