Genomic analysis of Vreelandella sp. F11 reveals its role in alginate utilization

IF 1.3 4区生物学 Q4 GENETICS & HEREDITY

Marine genomics Pub Date : 2024-11-21 DOI:10.1016/j.margen.2024.101160

Xin-Xiao Qu , Yu-Qi Zhang , Fei-Yu He , Xi-Ying Zhang , Peng Wang , Fei Xu , Yu-Qiang Zhang , Long-Sheng Zhao , Ping-Yi Li , Fang Zhao

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引用次数: 0

Abstract

Alginate, mainly produced by brown algae, is an important polysaccharide that supports the growth of marine bacteria. Vreelandella sp. F11 is a Gram-negative and aerobic marine bacterium, which was isolated from the brown algae sample collected from the Weihai coast, the Yellow Sea, China. Here, we present the complete genome of strain F11 and its genomic characteristics to utilize alginate. The genome of strain F11 comprises one circular chromosome with 4,840,724 bp and a GC content of 55.04 %. Strain F11 could grow with alginate as the sole carbon source. Genomic analysis revealed that strain F11 contains a gene cluster, encoding the enzymes, transporters and a regulator protein involved in utilizing alginate. Gene annotations suggested that the alginate utilization system of strain F11 is similar to those in Vibrio strains. However, strain F11 does not contain homologs of known KdgF (a key enzyme involved in the metabolism of alginate monomer) and inner membrane transporters of alginate oligomer uptake. The result indicated that strain F11 contains novel KdgF and inner membrane transporter system, which warrants further investigation. These data suggested that Vreelandella strains play a role in alginate utilization in the ocean and lay a foundation for the application of Vreelandella in the conversion of brown algae.

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对 Vreelandella sp. F11 的基因组分析揭示了其在海藻酸盐利用中的作用

藻酸盐主要由褐藻产生，是支持海洋细菌生长的重要多糖。F11 是一种革兰氏阴性好氧海洋细菌，从中国黄海威海沿岸采集的褐藻样品中分离得到。在此，我们展示了菌株 F11 的完整基因组及其利用海藻酸盐的基因组特征。菌株F11的基因组由一条环状染色体组成，共有4,840,724 bp，GC含量为55.04%。菌株 F11 能以海藻酸盐为唯一碳源生长。基因组分析表明，菌株 F11 含有一个基因簇，编码参与利用海藻酸的酶类、转运体和调节蛋白。基因注释表明，菌株 F11 的藻酸盐利用系统与弧菌菌株相似。然而，菌株 F11 不含已知的 KdgF（参与藻酸盐单体代谢的关键酶）和藻酸盐低聚物吸收内膜转运体的同源物。结果表明，菌株F11含有新型KdgF和内膜转运系统，值得进一步研究。这些数据表明，Vreelandella 菌株在海洋藻酸盐利用中发挥作用，为 Vreelandella 在褐藻转化中的应用奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Marine genomics 生物-遗传学

CiteScore

3.60

自引率

5.30%

发文量

审稿时长

29 days

期刊介绍： The journal publishes papers on all functional and evolutionary aspects of genes, chromatin, chromosomes and (meta)genomes of marine (and freshwater) organisms. It deals with new genome-enabled insights into the broader framework of environmental science. Topics within the scope of this journal include: • Population genomics and ecology • Evolutionary and developmental genomics • Comparative genomics • Metagenomics • Environmental genomics • Systems biology More specific topics include: geographic and phylogenomic characterization of aquatic organisms, metabolic capacities and pathways of organisms and communities, biogeochemical cycles, genomics and integrative approaches applied to microbial ecology including (meta)transcriptomics and (meta)proteomics, tracking of infectious diseases, environmental stress, global climate change and ecosystem modelling.