Multi-exposure image fusion for phase enhancement in digital holographic microscopy

IF 2.2 3区 物理与天体物理 Q2 OPTICS
Bingcai Liu , Xueling Zhang , Linlin Huang , Xinmeng Fang , Shaoping Ma , Xueliang Zhu , Hongjun Wang , Ailing Tian
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引用次数: 0

Abstract

Phase information in single exposure images is often lost for specimens with non-uniform transmittance. To address this issue, we propose a multi-exposure image fusion phase enhancement technique for holographic images. In this process, exposure time is varied to acquire 11 groups of four-step phase-shifted holographic images from a common sampling area. The resulting images are then decomposed using a wavelet transform. Maximum phase information from the low-frequency regions and regional features from the high-frequency regions were employed to avoid discontinuities in subsequent reconstructions, caused by regional truncation. Holographic images were then obtained after multi-exposure image fusion using a wavelet fusion method. Corresponding phase information was acquired by reconstructing fused holographic images using a four-step phase shifting technique. Experimental results showed that for specimens with non-uniform transmittance, this approach increased information entropy by 4.2%, edge density by 5%, and contrast by 3.8%, in comparison with the single-exposure digital holography phase reconstruction method. This result suggests that clarity and information content are improved, thereby enhancing the reconstructed phase.
用于数字全息显微镜相位增强的多曝光图像融合
对于透射率不均匀的试样,单次曝光图像中的相位信息往往会丢失。为解决这一问题,我们提出了全息图像的多曝光图像融合相位增强技术。在此过程中,通过改变曝光时间,从一个共同的采样区域获取 11 组四级相移全息图像。然后使用小波变换对得到的图像进行分解。采用低频区域的最大相位信息和高频区域的区域特征,以避免区域截断造成后续重建的不连续性。然后,利用小波融合方法进行多曝光图像融合,获得全息图像。利用四步移相技术对融合后的全息图像进行重建,从而获得相应的相位信息。实验结果表明,与单次曝光数字全息相位重建方法相比,对于透射率不均匀的试样,这种方法可使信息熵提高 4.2%,边缘密度提高 5%,对比度提高 3.8%。这一结果表明,清晰度和信息含量都得到了提高,从而增强了重建的相位。
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来源期刊
Optics Communications
Optics Communications 物理-光学
CiteScore
5.10
自引率
8.30%
发文量
681
审稿时长
38 days
期刊介绍: Optics Communications invites original and timely contributions containing new results in various fields of optics and photonics. The journal considers theoretical and experimental research in areas ranging from the fundamental properties of light to technological applications. Topics covered include classical and quantum optics, optical physics and light-matter interactions, lasers, imaging, guided-wave optics and optical information processing. Manuscripts should offer clear evidence of novelty and significance. Papers concentrating on mathematical and computational issues, with limited connection to optics, are not suitable for publication in the Journal. Similarly, small technical advances, or papers concerned only with engineering applications or issues of materials science fall outside the journal scope.
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