Decoding differentially expressed genes to identify potential immunity associated biomarkers in Tuberculosis: An integrative bioinformatics approach

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2024-11-22 DOI:10.1016/j.bbrep.2024.101870

Ankur Datta, Divyanshi Gupta, Diya Waryani, George Priya Doss C

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Abstract

Tuberculosis (TB) poses a significant threat to the Indian population, with India accounting for 20 % of the global TB cases. The current study aims to identify molecular biomarkers for better diagnostics by comparing the transcriptome signatures of healthy individuals against TB-affected individuals. Next-generation sequencing (NGS) tools were used to identify critical differentially expressed genes (DEGs). 302 DEGs were identified based on a logFC threshold of |3| and adjusted p-value < 0.05. STRING database was used to plot the interactions amongst the 302 DEGs. The DEGs were functionally annotated, highlighting numerous physiological functions affected due to the dysregulation of the identified hub genes. TLR4, FCGR1A, ITGAM, LTF, and CXCR2 were the hub genes identified and observed to dysregulate crucial physiological functions. TLR4 has been implicated in the progression of TB in various populations, and the findings of this study will enable researchers to improve the current landscape of diagnostics for TB.

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解码差异表达基因，识别结核病中潜在的免疫相关生物标志物：综合生物信息学方法

肺结核（TB）对印度人口构成了严重威胁，印度的肺结核病例占全球的 20%。目前的研究旨在通过比较健康人和受结核病影响的人的转录组特征来确定分子生物标志物，以便更好地进行诊断。研究使用了下一代测序（NGS）工具来识别关键的差异表达基因（DEGs）。根据 logFC |3|阈值和调整后的 p 值 < 0.05，确定了 302 个 DEGs。STRING 数据库用于绘制 302 个 DEGs 之间的相互作用图。对 DEGs 进行了功能注释，突出了由于已确定的枢纽基因失调而影响的众多生理功能。TLR4、FCGR1A、ITGAM、LTF 和 CXCR2 是已确定的枢纽基因，并观察到它们对关键生理功能的失调。TLR4 与不同人群中结核病的进展有牵连，这项研究的发现将使研究人员能够改善目前结核病的诊断方法。

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.