Evaluating the role of biopriming and nanopriming on the morphometric, biochemical, and yield parameters of Chickpea (Cicer arietinum L.) under drought stress

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2024-11-13 DOI:10.1016/j.stress.2024.100675

Simran Rani , Priyanka Dahiya , Aarzoo Sharma , Yash Vashisth , Kiran Arora , Amita Suneja Dang , Pooja Suneja

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引用次数: 0

Abstract

This study evaluated drought mitigation potential of biopriming with plant growth promoting endophytic bacteria (PGPEBs) and nanopriming with their biological copper nanoparticles (CuNPs) and chemical CuNPs under polyethylene glycol (PEG-6000) induced moderate (MD-5%) and severe drought (SD-10%) in chickpea (Cicer arietinum L.). The crop harvested at 15 DDS (Days to drought stress) was analysed for morphometric and biochemical parameters of drought tolerant (DT), BG-4958 and drought sensitive (DS), ICC-1882 chickpea varieties. In morphometric traits of DT variety, N₁X led 384% increase in shoot dry weight (SDW) under MD while B₂ in root dry weight (RDW) under SD (418%). For DS variety, N₂X led 444% and 727% increase in SDW (MD) and RDW (SD), respectively. Amongst biochemical parameters, maximum increment was noticed in total chlorophyll content (TCC) by B₁ under MD (703%) as well as SD (1206%) in DT variety. B₁ also led highest increment (758%) in TCC of DS variety under SD while B₂ under MD (300%). B₂ resulted in 242% increment in total soluble carbohydrates (TSC) and 47% increase in total protein content (TPC) of DS variety under SD. N₁X and N₁Y led 318% and 100% increase in the activity of ascorbate peroxidase (APX) and peroxidase (POD) of DS variety. This variety exhibiting pronounced response was subjected to correlation analysis revealing highest correlation amongst morpho-biochemical traits under SD. Score plot in principal component analysis (PCA) of DS variety showed that biopriming and N₁X having higher score values for PC₂ mainly influenced by biochemical parameters also improved the yield parameters to a greater extent as analyzed on 120 DDS. Harvesting index, the ultimate indicator of the agricultural output remained insignificant in DT variety. On the other hand, B₂ and N₁X led highest harvesting indices under MD (94%) and SD (69%), respectively, in DS variety, owing to their higher grain yield than biological yield and higher score values influencing biochemical parameters under stress. The present study provides insights into the beneficial role of PGPEBs and biosynthesized CuNPs in mitigating the adverse effects of drought in chickpea.

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评估生物riming 和纳米riming 对干旱胁迫下鹰嘴豆（Cicer arietinum L.）形态计量、生物化学和产量参数的作用

本研究评估了在聚乙二醇（PEG-6000）诱导的鹰嘴豆（Cicer arietinum L.）中度干旱（MD-5%）和重度干旱（SD-10%）条件下，利用植物生长促进内生细菌（PGPEBs）进行生物riming，以及利用其生物纳米铜颗粒（CuNPs）和化学纳米铜颗粒进行纳米riming的抗旱潜力。在 15 DDS（干旱胁迫天数）收获的作物中，对耐旱（DT）鹰嘴豆品种 BG-4958 和对干旱敏感（DS）鹰嘴豆品种 ICC-1882 的形态和生化参数进行了分析。在 DT 品种的形态特征方面，在 MD 条件下，N1X 使芽干重（SDW）增加了 384%，而在 SD 条件下，B2 使根干重（RDW）增加了 418%。对于 DS 品种，N2X 使 SDW（MD）和 RDW（SD）分别增加了 444% 和 727%。在生化参数中，B1 在 DT 品种的 MD（703%）和 SD（1206%）条件下，叶绿素总含量（TCC）的增幅最大。B1 也导致在 SD 条件下 DS 品种总叶绿素含量的最高增幅（758%），而 B2 则导致在 MD 条件下总叶绿素含量的最高增幅（300%）。在自交条件下，B2 使 DS 品种的总可溶性碳水化合物（TSC）增加了 242%，总蛋白质含量（TPC）增加了 47%。N1X 和 N1Y 使 DS 品种的抗坏血酸过氧化物酶（APX）和过氧化物酶（POD）的活性分别提高了 318% 和 100%。对这一反应明显的品种进行相关性分析后发现，在自毁条件下，其形态生化性状之间的相关性最高。DS 品种的主成分分析（PCA）得分图显示，生物riming 和 N1X 在 PC2 中的得分值较高，主要受生化参数的影响。作为农业产量的最终指标，收获指数在 DT 品种中仍然不显著。另一方面，B2 和 N1X 在 MD（94%）和 SD（69%）条件下的收获指数在 DS 品种中分别最高，这是因为它们的谷物产量高于生物产量，并且在胁迫条件下影响生化参数的分值较高。本研究揭示了 PGPEBs 和生物合成的 CuNPs 在减轻鹰嘴豆干旱不利影响方面的有益作用。

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.