Quality and yield of potato seed tubers as influenced by plant growth promoting rizobacteria

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Muhammad Raqib Rasul, Tavga Sulaiman Rashid
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引用次数: 0

Abstract

Using chemical fertilizers in agriculture increases production and improves the quality of the product; however, their higher usage globally has brought forth damage to ecosystems. Using biofertilizers is a better strategy to reduce the use of chemical fertilizers and ultimately increase soil fertility. This study aimed to isolate, identify, and characterize bacteria from the soil rhizosphere of medicinal plants (Rumex tuberosus L. and Verbascum sp.) for in vivo screening. Nitrogen fixation, phosphate solubilization, HCN, ammonia levels, Lipase, protease, catalase and siderophore production biochemical tests were also conducted. The two isolates that gave positive results from the biochemical tests were chosen out of 25 for further experiments. Based on 16S rRNA sequencing analysis the isolated organisms were identified as Alcaligenes faecalis Go1 (Accession No. OP001725) and Bacillus subtilis T11 (Accession No. OP218376). The compound fertilizer NPK was used as the positive control for field experiments, while selected stains were individually and in-combination were tested on potato crops as inoculum, over two successive cropping seasons. Plant height, number of tubers per plant, chlorophyll content, and tuber weight all increased for both isolated bacterial strains. The quality of the potato tubers was checked through visual observation for the presence or absence of disease symptoms. The treated tubers exhibited excellent quality, remaining free from any signs of disease, however, the control tubers showed infections with (Streptomyces scabiei, Fusarium sp., F. solani and Erwinia amylovora). The soil analyzed after harvesting both bacteria increased percentages of P, Ca2+, Mg2+, Na+, K+, SO4, total nitrogen content and total organic matter. The findings showed that the tested bacterial isolates could replace the use of chemical fertilizers in the production of potatoes.
马铃薯块茎种子的质量和产量受植物生长促进有益菌的影响
在农业生产中使用化肥可以提高产量和产品质量,但化肥在全球范围内的大量使用却对生态系统造成了破坏。使用生物肥料是减少化肥使用并最终提高土壤肥力的更好策略。本研究旨在从药用植物(Rumex tuberosus L. 和 Verbascum sp.)的土壤根圈中分离、鉴定和描述细菌,以进行体内筛选。此外,还进行了固氮、磷酸盐溶解、HCN、氨含量、脂肪酶、蛋白酶、过氧化氢酶和苷元生成等生化测试。从 25 个分离株中选出生化测试结果呈阳性的两个进行进一步实验。根据 16S rRNA 测序分析,确定分离出的生物为粪钙杆菌 Go1(登录号:OP001725)和枯草芽孢杆菌 T11(登录号:OP218376)。田间试验以氮磷钾复合肥为阳性对照,同时在连续两个耕种季节对所选污点作为接种物单独或混合在马铃薯作物上进行试验。两种分离出的细菌菌株的植株高度、每株块茎数、叶绿素含量和块茎重量都有所增加。马铃薯块茎的质量是通过肉眼观察是否有病害症状来检验的。经过处理的块茎表现出极佳的品质,没有任何病害迹象,但对照组块茎则出现了(疥癣链霉菌、镰刀菌属 Fusarium sp.、F. solani 和 Erwinia amylovora)感染。收获后分析的土壤中,两种细菌都增加了 P、Ca2+、Mg2+、Na+、K+、SO4、全氮含量和有机物总量的百分比。研究结果表明,在马铃薯生产中,测试的细菌分离物可以替代化肥的使用。
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来源期刊
Biocatalysis and agricultural biotechnology
Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
7.70
自引率
2.50%
发文量
308
审稿时长
48 days
期刊介绍: Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.
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