Reconnoitring antiepileptic and anxiolytic effects of Indian herbs: Findings from zebrafish behavioral research

Abstract

Introduction

In the 21st century, neuropsychological disorders such as anxiety, insomnia, and epilepsy have become increasingly prevalent due to irregular lifestyles, unhealthy food habits, and work pressure. These disorders significantly impact individuals' emotional, mental, and physical well-being, leading to decreased functionality and potential long-term health complications.

Materials and methods

The research project utilized zebrafish behavioral studies to investigate the effects of herbal extracts on anxiety-like behavior and stress levels. Specifically, the study focused on four herbal plants, Withania somnifera, Adiantumcapillus-veneris, Marsileaq uadrifolia Linn. and Nardostachys jatamansi, to assess their potential in managing neuropsychological disorders. The zebrafish were exposed to solutions or extracts of these plants, and standard doses of pentylenetetrazol (PTZ) were administered to induce seizures for anti-seizure tests. Additionally, six behavioural studies, including the Novel Tank Test and Open Field Test, were conducted to evaluate anxiety-like behaviour. The methods employed in this study aimed to provide insights into the potential anxiolytic and stress-reducing properties of the herbal extracts.

Results

The findings reveal that Withania somnifera and Marsilea quadrifolia Linn., demonstrate promising effects in reducing anxiety-like behaviour and stress levels in zebrafish compared toAdiantumcapillus-veneris and Nardostachys jatamansi. These results also show the potential to improve explorative behavior and reduce time spent in dark areas, indicating anxiolytic properties.

Conclusion

The study highlights the potential of herbal therapy in managing neuropsychological disorders and emphasizes the importance of exploring alternative treatment approaches. The results provide valuable insights into the potential use of Withania somnifera and Marsilea quadrifolia Linn extracts in alleviating anxiety and stress-related behaviours and mitigating seizures, paving the way for further research and potential therapeutic applications.