Chang Lu, Shiyi Liao, Baode Chen, Li Xu, Na Wu, Dingyou Lu, Heemin Kang, Xiao-Bing Zhang, Guosheng Song
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引用次数: 0
Abstract
Nitric oxide (NO), a pivotal signalling molecule, plays multifaceted roles in physiological and pathological processes, including cardiovascular and immune functions, neurotransmission and cancer progression. Nevertheless, measuring NO in vivo is challenging due to its transient nature and the complexity of the biological environment. Here we describe NO-responsive magnetic probes made of crosslinked superparamagnetic iron oxide nanoparticles tethered to a NO-sensitive cleavable linker for highly sensitive and selective NO magnetic resonance imaging in vivo. These probes enable the detection of NO at concentrations as low as 0.147 μM, allowing for the imaging and quantification of NO in mouse tumour models, studying its effects on tumour progression and immunity and assessing the response of tumour-associated macrophages to cancer immunotherapeutic agents. Additionally, they facilitate concurrent anatomical and molecular imaging of organs, helping to identify pathological alterations in the liver. Overall, these probes represent promising non-invasive tools for investigating the dose-dependent conflicting role of NO in physiological and pathophysiological processes.
一氧化氮(NO)是一种关键的信号分子,在生理和病理过程中发挥着多方面的作用,包括心血管和免疫功能、神经传递和癌症进展。然而,由于氮氧化物的瞬时性和生物环境的复杂性,在体内测量氮氧化物具有挑战性。在这里,我们描述了由交联超顺磁性氧化铁纳米粒子和对 NO 敏感的可裂解连接体组成的 NO 响应磁性探针,用于在体内进行高灵敏度和选择性 NO 磁共振成像。这些探针能够检测低至 0.147 μM 浓度的 NO,从而能够对小鼠肿瘤模型中的 NO 进行成像和量化,研究 NO 对肿瘤进展和免疫的影响,并评估肿瘤相关巨噬细胞对癌症免疫治疗药物的反应。此外,这些探针还有助于同时对器官进行解剖和分子成像,帮助确定肝脏的病理改变。总之,这些探针是研究 NO 在生理和病理生理过程中的剂量依赖性冲突作用的很有前途的非侵入性工具。
期刊介绍:
Nature Materials is a monthly multi-disciplinary journal aimed at bringing together cutting-edge research across the entire spectrum of materials science and engineering. It covers all applied and fundamental aspects of the synthesis/processing, structure/composition, properties, and performance of materials. The journal recognizes that materials research has an increasing impact on classical disciplines such as physics, chemistry, and biology.
Additionally, Nature Materials provides a forum for the development of a common identity among materials scientists and encourages interdisciplinary collaboration. It takes an integrated and balanced approach to all areas of materials research, fostering the exchange of ideas between scientists involved in different disciplines.
Nature Materials is an invaluable resource for scientists in academia and industry who are active in discovering and developing materials and materials-related concepts. It offers engaging and informative papers of exceptional significance and quality, with the aim of influencing the development of society in the future.