Empagliflozin improves sperm quality in diabetic rats by lowering oxidative stress and reducing apoptosis-related genes expression

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology Pub Date : 2024-11-23 DOI:10.1016/j.repbio.2024.100971

Mina Kiani, Malek Soleimani Mehranjani, Mohammad Ali Shariatzadeh

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引用次数: 0

Abstract

Type 2 diabetes mellitus (T2DM) negatively impacts sperm health by increasing oxidative stress. Empagliflozin, a type of sodium-glucose cotransporter 2 (SGLT2) inhibitor, not only reduces blood glucose levels in T2DM but also has antioxidant properties. This study aimed to investigate the antioxidant effects of empagliflozin on sperm quality and spermatogenesis indices in rats with T2DM.Male rats were divided into three groups (n = 6): control, diabetes mellitus (DM, induced by streptozotocin + nicotinamide), and DM treated with empagliflozin (10 mg/kg, 56 days, via gavage). Sperm parameters including sperm count, viability, motility, normal morphology, DNA integrity, maturation, tail length, and daily sperm production were analyzed. Additionally, spermatogenesis indices, Bax/Bcl2 ratio and serum levels of superoxide dismutase (SOD), total antioxidant capacity (TAC), and malondialdehyde (MDA) were measured. In the DM group, there was a significant decrease in the mean sperm parameters, SOD, TAC, and spermatogenesis indices, along with a significant increase in the percentage of single-stranded DNA (AO⁺), immature sperm nuclei (AB⁺), MDA levels, and Bax/Bcl2 ratio, compared to the control group. In the DM + empagliflozin group, there was a significant increase in the mean sperm parameters, SOD levels (P˂0.001), and spermatogenesis indices. Additionally, there was a significant decrease in the mean AB+ (P˂0.01), AO⁺, MDA levels (P˂0.001), and Bax/Bcl2 ratio compared to the DM group. The results showed that empagliflozin can mitigate the adverse effects of diabetes and improve sperm quality and increase sperm production in diabetic rats.

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Empagliflozin 可通过降低氧化应激和减少细胞凋亡相关基因的表达来改善糖尿病大鼠的精子质量。

2 型糖尿病（T2DM）会增加氧化应激，从而对精子健康产生负面影响。Empagliflozin 是一种钠-葡萄糖共转运体 2（SGLT2）抑制剂，它不仅能降低 T2DM 患者的血糖水平，还具有抗氧化作用。雄性大鼠分为三组（n = 6）：对照组、由链脲佐菌素+烟酰胺诱导的糖尿病组（DM）和经empagliflozin（10 mg/kg，56天，灌胃）治疗的DM组。分析了精子参数，包括精子数量、存活率、活力、正常形态、DNA完整性、成熟度、尾长和每日精子产量。此外，还测定了精子发生指数、Bax/Bcl2 比率以及血清中超氧化物歧化酶（SOD）、总抗氧化能力（TAC）和丙二醛（MDA）的水平。与对照组相比，DM组的平均精子参数、SOD、TAC和精子发生指数显著下降，单链DNA（AO+）、未成熟精子核（AB+）、MDA水平和Bax/Bcl2比值显著增加。在DM+empagliflozin组中，平均精子参数、SOD水平（P˂0.001）和精子发生指数显著增加。此外，与DM组相比，AB+（P˂0.01）、AO+、MDA水平（P˂0.001）和Bax/Bcl2比值均明显下降。结果表明，empagliflozin能减轻糖尿病的不良影响，改善糖尿病大鼠的精子质量，提高精子产量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive biology 生物-生殖生物学

CiteScore

3.90

自引率

0.00%

发文量

审稿时长

29 days

期刊介绍： An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn, Poland. Reproductive Biology is an international, peer-reviewed journal covering all aspects of reproduction in vertebrates. The journal invites original research papers, short communications, review articles and commentaries dealing with reproductive physiology, endocrinology, immunology, molecular and cellular biology, receptor studies, animal breeding as well as andrology, embryology, infertility, assisted reproduction and contraception. Papers from both basic and clinical research will be considered.